1887

Abstract

Strain 15-4, a Gram-stain-negative, rod-shaped, non-motile bacterial strain that produced flexirubin-type pigments, was isolated from Tibet Province, China, and characterized by using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the family , phylum ‘’, and was related to members of the genus , with sequence similarities ranging from 94.1 to 96.4 %. Strain 15-4 contained MK-7 as the predominant menaquinone and its DNA GC content was 46.9 mol%. The major fatty acids of strain 15-4 were iso-C (41.3 %), iso-C G (14.9 %), iso-C 3-OH (13.2 %) and summed feature 3 (comprising Cω7 and/or iso-C 2-OH, 16.0 %). These chemotaxonomic data supported the affiliation of strain 15-4 to the genus . However, a number of physiological and biochemical features enabled the isolate to be differentiated phenotypically from recognized speciesof the genus . On the basis of the evidence presented, it is proposed that strain 15-4 represents a novel species, sp. nov.; the type strain is 15-4 ( = CCTCC AB 209167 = NRRL B-59394). On the basis of these data, an emended description of the genus is also proposed.

Funding
This study was supported by the:
  • , Ministry of Science and Technology of the People’s Republic of China , (Award 2005DKA21208)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.022103-0
2011-05-01
2021-03-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/5/1201.html?itemId=/content/journal/ijsem/10.1099/ijs.0.022103-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
    [Google Scholar]
  2. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Kim B. Y., Weon H.-Y., Yoo S.-H., Hong S.-B., Kwon S.-W., Stackebrandt E., Go S.-J. 2007; Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. Int J Syst Evol Microbiol 57:538–541 [CrossRef][PubMed]
    [Google Scholar]
  4. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  5. Kumar S., Tamura K., Nei M. 2004; mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef][PubMed]
    [Google Scholar]
  6. Lane D. J. 1991; 16S–23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 125–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  7. Ludwig, W., Euzéby, J. & Whitman, W. B. (2008). Draft Taxonomic Outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi and Gemmatimonadetes for Volume 4 of the second edition of Bergey’s Manual of Systematic Bacteriology. ( http://www.bergeys.org/outlines.html)
  8. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  9. Reichenbach H. 1992; The order Cytophagales . In The Prokaryotes, 2nd edn. vol. 4 pp. 3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [CrossRef]
    [Google Scholar]
  10. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Woods W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  12. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  13. Wang H., Zhang Y. Z., Man C. X., Chen W. F., Sui X. H., Li Y., Zhang X. X., Chen W. X. 2009; Niabella yanshanensis sp. nov., isolated from the soybean rhizosphere. Int J Syst Evol Microbiol 59:2854–2856 [CrossRef][PubMed]
    [Google Scholar]
  14. Weon H. Y., Kim B.-Y., Joa J.-H., Kwon S.-W., Kim W.-G., Koo B.-S. 2008; Niabella soli sp. nov., isolated from soil from Jeju Island, Korea. Int J Syst Evol Microbiol 58:467–469 [CrossRef][PubMed]
    [Google Scholar]
  15. Weon H. Y., Yoo S.-H., Kim B.-Y., Son J.-A., Kim Y.-J., Kwon S.-W. 2009; Niabella ginsengisoli sp. nov., isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 59:1282–1285 [CrossRef][PubMed]
    [Google Scholar]
  16. Xie C. H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.022103-0
Loading
/content/journal/ijsem/10.1099/ijs.0.022103-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error