1887

Abstract

A novel actinomycete strain, designated YIM 63587, was isolated from surface-sterilized roots of L. collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 63587 was affiliated to the genus . 16S rRNA gene sequence similarities between strain YIM 63587 and type strains of species of the genus were 96.6–93.8 %. The diagnostic cell-wall diamino acid in the peptidoglycan layer of strain YIM 63587 was -diaminopimelic acid and the whole-cell sugars were arabinose, galactose, mannose and ribose. The predominant menaquinone was MK-8(H) (97.7 %). The phospholipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol mannosides, phosphatidylinositol and an unknown phospholipid. The major cellular fatty acids (>5 %) were iso-C (44.7 %), iso-C (10.3 %), iso-C H (9.8 %) and iso-C (7.7 %). The G+C content of the genomic DNA was 68.2 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain YIM 63587 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 63587 ( = DSM 45313 = CCTCC AA 208081).

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2011-05-01
2020-01-26
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Supplements

vol. , part 5, pp. 1061 - 1065

Maximum-parsimony tree based on 16S rRNA gene sequences showing the relationships between sp. nov. YIM 63587 and other members of the genus .

Maximum-likelihood tree based on 16S rRNA gene sequences showing the relationships between sp. nov. YIM 63587 and other members of the genus .

Polar lipid composition of sp. nov. YIM 63587 after growth on ISP 2.

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