1887

Abstract

A novel actinomycete strain, designated YIM 63587, was isolated from surface-sterilized roots of L. collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 63587 was affiliated to the genus . 16S rRNA gene sequence similarities between strain YIM 63587 and type strains of species of the genus were 96.6–93.8 %. The diagnostic cell-wall diamino acid in the peptidoglycan layer of strain YIM 63587 was -diaminopimelic acid and the whole-cell sugars were arabinose, galactose, mannose and ribose. The predominant menaquinone was MK-8(H) (97.7 %). The phospholipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol mannosides, phosphatidylinositol and an unknown phospholipid. The major cellular fatty acids (>5 %) were iso-C (44.7 %), iso-C (10.3 %), iso-C H (9.8 %) and iso-C (7.7 %). The G+C content of the genomic DNA was 68.2 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain YIM 63587 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 63587 ( = DSM 45313 = CCTCC AA 208081).

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833800)
  • National Natural Science Foundation of China (Award U0932601)
  • Scientific Research Foundation of Yunnan Educational Commission (Award 09J0038)
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2011-05-01
2021-07-29
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