1887

Abstract

Three ustilaginomycetous anamorphic strains were isolated from flowers of in mangrove forests of Taiwan. Phylogenetic analyses based on the combined sequences of internal transcribed spacer 1 (ITS1)-5.8S-ITS2 and the D1/D2 domain of the large-subunit (LSU) rDNA indicated that the closest recognized species was . The results of a DNA–DNA hybridization experiment and the physiological characteristics showed that the three strains represent a novel species within the genus . The name sp. nov. is proposed, with FIRDI 007 (=BCRC 23165 =CBS 11676) as the type strain. In addition, based on phenotypic characteristics and the phylogenetic analyses of the combined sequences of the ITS region and D1/D2 domain of the LSU rDNA, was clustered with the genus . A new combination, comb. nov. (type strain LM418 =DSM 18755 =ATCC MYA-4092 =NRRL Y-48466 =CBS 10858 =BCRC 23177), is proposed.

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2011-02-01
2024-04-18
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References

  1. Begerow D., Bauer R., Oberwinkler F. 2001; Muribasidiospora : Microstromatales or Exobasidiales ?. Mycol Res 105:798–810 [CrossRef]
    [Google Scholar]
  2. Begerow D., Stoll M., Bauer R. 2006; A phylogenetic hypothesis of Ustilaginomycotina based on multiple gene analyses and morphological data. Mycologia 98:906–916 [CrossRef]
    [Google Scholar]
  3. de Beer Z. W., Begerow D., Bauer R., Pegg G. S., Crous P. W., Wingfield M. J. 2006; Phylogeny of the Quambalariaceae fam. nov., including important Eucalyptus pathogens in South Africa and Australia. Stud Mycol 55:289–298 [CrossRef]
    [Google Scholar]
  4. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [CrossRef]
    [Google Scholar]
  5. Kurtzman C. P. 1998; Nuclear DNA hybridization: quantitation of close genetic relationships. In The Yeasts, a Taxonomic Study, 4th edn. pp 63–68 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
  6. Lee F.-L., Lee C.-F., Okada S., Uchimura T., Komagata K., Kozaki M. 1993; Candida galacta comb. nov., a new combination for Candida apis var. galacta . Int J Syst Bacteriol 43:183–184 [CrossRef]
    [Google Scholar]
  7. Lee F.-L., Fu H.-M., Hsu W.-H. 1998; DNA hybridization and electrokaryotype study of some Candida species. Int J Syst Bacteriol 48:1463–1466 [CrossRef]
    [Google Scholar]
  8. Mahdi L. E., Statzell-Tallman A., Fell J. W., Brown M. V., Donachie S. P. 2008 Sympodiomycopsis lanaiensis sp. nov., a basidiomycetous yeast (Ustilaginomycotina: Microstromatales) from marine driftwood in Hawai'i. FEMS Yeast Res 8, 1357–1363 [CrossRef]
  9. Nagahama T., Hamamoto M., Nakase T., Horikoshi K. 1999; Kluyveromyces nonfermentans sp. nov., a new yeast species isolated from the deep sea. Int J Syst Bacteriol 49:1899–1905 [CrossRef]
    [Google Scholar]
  10. O'Donnell K. 1992; Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum ( Gibberella pulicaris ). Curr Genet 22:213–220 [CrossRef]
    [Google Scholar]
  11. Sipiczki M., Kajdacsi E. 2009; Jaminaea angkorensis gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales. Int J Syst Evol Microbiol 59:914–920 [CrossRef]
    [Google Scholar]
  12. Sugita T., Nishikawa A., Ikeda R., Shinoda T. 1999a; Identification of medically relevant Trichosporon species based on sequences of internal transcribed spacer regions and construction of a database for Trichosporon identification. J Clin Microbiol 37:1985–1993
    [Google Scholar]
  13. Sugita T., Cañete-Gibas C., Takashima M., Nakase T. 1999b; Three new species of Bullera isolated from leaves in the Ogasawara Islands. Mycoscience 40:491–501 [CrossRef]
    [Google Scholar]
  14. Sugiyama J., Tokuoka K., Suh S.-O., Hirata A., Komagata K. 1991; Sympodiomycopsis : a new yeast-like anamorph genus with basidiomycetous nature from orchid nectar. Antonie van Leeuwenhoek 59:95–108 [CrossRef]
    [Google Scholar]
  15. Suh S.-O., Sugiyama J. 1994; Phylogenetic placement of the basidiomycetous yeasts Kondoa malvinella and Rhodosporidium dacryoidum , and the anamorphic yeast Sympodiomycopsis paphiopedili by means of 18S rRNA gene sequence analysis. Mycoscience 35:367–375 [CrossRef]
    [Google Scholar]
  16. Suh S.-O., Hirata A., Sugiyama J., Komagata K. 1993; Septal ultrastructure of basidiomycetous yeasts and their taxonomic implications with observations on the ultrastructure of Erythrobasidium hasegawianum and Sympodiomycopsis paphiopedili . Mycologia 85:30–37 [CrossRef]
    [Google Scholar]
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  20. White T. J., Bruns T., Lee S., Taylor J. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications pp 315–322 Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. San Diego: Academic Press;
    [Google Scholar]
  21. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn. pp 77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
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