In the course of a polyphasic study it was observed that ‘Dactylosporangium variesporum’ NRRL B-16296 is misclassified in the genus Dactylosporangium as it exhibits properties consistent with its assignment to the genus Saccharothrix. Phylogenetic analyses based on 16S rRNA gene sequences show that the strain falls within the evolutionary radiation of the genus Saccharothrix, a result which is supported by corresponding chemotaxonomic and morphological markers. The strain is phylogenetically most closely, albeit loosely, related to Saccharothrix espanaensis, but can be readily distinguished from this and other species of the genus Saccharothrix with validly described names by using a range of phenotypic properties. The combined genotypic and phenotypic data demonstrate conclusively that this strain should be classified as a new species in the genus Saccharothrix for which the name Saccharothrix variisporea sp. nov. is proposed. The type strain is NRRL B-16296T (=ATCC 31203T =DSM 43911T =JCM 3273T =NBRC 14104T).
CollinsM. D.1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp 265–309 Edited by
GoodfellowM.,
O'DonnellA. G.
Chichester: Wiley;
GonzalezJ. M.,
Saiz-JimenezC.2005; A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. Extremophiles 9:75–79[CrossRef]
JonesK. L.1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
JukesT. H.,
CantorC. R.1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol 3 pp 21–132 Edited by
MunroH. N.
New York: Academic Press;
LabedaD. P.2002; The family Actinosynnemataceae
. In The Prokaryotes, 3rd edn. pp 654–668 Edited by
DworkinM.,
FalkowS.,
RosenbergE.,
SchleiferK.,
StackebrandtE.
New York: Springer;
LabedaD. P.,
KroppenstedtR. M.2007; Proposal of Umezawaea gen. nov., a new genus of the Actinosynnemataceae related to Saccharothrix , and transfer of Saccharothrix tangerinus Kinoshita et al. 2000 as Umezawaea tangerina gen. nov., comb. nov. Int J Syst Evol Microbiol 57:2758–2761[CrossRef]
LabedaD. P.,
LechevalierM. P.1989; Amendment of the genus Saccharothrix Labeda et al. 1984 and descriptions ofSaccharothrix espanaensis sp. nov., Saccharothrix cryophilis sp. nov., and Saccharothrix mutabilis comb. nov. Int J Syst Bacteriol 39:420–423[CrossRef]
LabedaD. P.,
TestaR. T.,
LechevalierM. P.,
LechevalierH. A.1984; Saccharothrix : a new genus of the Actinomycetales related to Nocardiopsis
. Int J Syst Bacteriol 34:426–431[CrossRef]
LechevalierM. P.,
LechevalierH.1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443[CrossRef]
MinnikinD. E.,
AlshamaonyL.,
GoodfellowM.1975; Differentiation of Mycobacterium, Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204[CrossRef]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241[CrossRef]
SchaalK. P.1985; Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods in Bacterial Systematics pp 359–381 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
TakahashiK.,
NeiM.2000; Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17:1251–1258[CrossRef]
TanG. Y. A.,
WardA. C.,
GoodfellowM.2006; Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media. Syst Appl Microbiol 29:557–569[CrossRef]
UchidaK.,
SeinoA.1997; Intra- and intergeneric relationships of various actinomycete strains based on the acyl types of the muramyl residue in cell wall peptidoglycans examined in a glycolate test. Int J Syst Bacteriol 47:182–190[CrossRef]
UchidaK.,
KudoT.,
SuzukiK.,
NakaseT.1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]