1887

Abstract

In the course of a polyphasic study it was observed that ‘’ NRRL B-16296 is misclassified in the genus as it exhibits properties consistent with its assignment to the genus . Phylogenetic analyses based on 16S rRNA gene sequences show that the strain falls within the evolutionary radiation of the genus , a result which is supported by corresponding chemotaxonomic and morphological markers. The strain is phylogenetically most closely, albeit loosely, related to but can be readily distinguished from this and other species of the genus with validly described names by using a range of phenotypic properties. The combined genotypic and phenotypic data demonstrate conclusively that this strain should be classified as a new species in the genus for which the name sp. nov. is proposed. The type strain is NRRL B-16296 (=ATCC 31203 =DSM 43911 =JCM 3273 =NBRC 14104).

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2011-02-01
2024-10-03
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References

  1. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp 265–309 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  2. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  3. Gonzalez J. M., Saiz-Jimenez C. 2005; A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. Extremophiles 9:75–79 [CrossRef]
    [Google Scholar]
  4. Goodfellow M., Brown A. B., Cai J., Chun J., Collins M. D. 1997; Amycolatopsis japonicum sp. nov., an actinomycete producing (S,S)-N,N′-ethylenediaminedisuccinic acid. Syst Appl Microbiol 20:78–84 [CrossRef]
    [Google Scholar]
  5. Hu Y.-T., Zhou P.-J., Zhou Y.-G., Liu Z.-H., Liu S.-J. 2004; Saccharothrix xinjiangensis sp. nov., a pyrene-degrading actinomycete isolated from Tianchi Lake, Xinjiang, China. Int J Syst Evol Microbiol 54:2091–2094 [CrossRef]
    [Google Scholar]
  6. Jeon Y.-S., Chung H., Park S., Hur I., Lee J.-H., Chun J. 2005; jphydit: a java-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [CrossRef]
    [Google Scholar]
  7. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  10. Labeda D. P. 2002; The family Actinosynnemataceae . In The Prokaryotes, 3rd edn. pp 654–668 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K., Stackebrandt E. New York: Springer;
    [Google Scholar]
  11. Labeda D. P., Kroppenstedt R. M. 2007; Proposal of Umezawaea gen. nov., a new genus of the Actinosynnemataceae related to Saccharothrix , and transfer of Saccharothrix tangerinus Kinoshita et al. 2000 as Umezawaea tangerina gen. nov., comb. nov. Int J Syst Evol Microbiol 57:2758–2761 [CrossRef]
    [Google Scholar]
  12. Labeda D. P., Lechevalier M. P. 1989; Amendment of the genus Saccharothrix Labeda et al. 1984 and descriptions ofSaccharothrix espanaensis sp. nov., Saccharothrix cryophilis sp. nov., and Saccharothrix mutabilis comb. nov. Int J Syst Bacteriol 39:420–423 [CrossRef]
    [Google Scholar]
  13. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A. 1984; Saccharothrix : a new genus of the Actinomycetales related to Nocardiopsis . Int J Syst Bacteriol 34:426–431 [CrossRef]
    [Google Scholar]
  14. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  15. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  16. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  18. O'Donnell A. G., Falconer C., Goodfellow M., Ward A. C., Williams E. 1993; Biosystematics and diversity amongst novel carboxydotrophic actinomycetes. Antonie van Leeuwenhoek 64:325–340
    [Google Scholar]
  19. Otoguro M., Tamura T., Suzuki K., Hayakawa M. 2009; Saccharothrix violaceirubra sp. nov., isolated from soil and plant litter. Int J Syst Evol Microbiol 59:1504–1507 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  22. Schaal K. P. 1985; Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods in Bacterial Systematics pp 359–381 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  23. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  24. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  25. Takahashi K., Nei M. 2000; Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17:1251–1258 [CrossRef]
    [Google Scholar]
  26. Tan G. Y. A., Ward A. C., Goodfellow M. 2006; Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media. Syst Appl Microbiol 29:557–569 [CrossRef]
    [Google Scholar]
  27. Tomita K., Kobaru S., Hanada M., Tsukiura H. 1977; Fermentation process. U.S Patent, 4026766, May 31
    [Google Scholar]
  28. Uchida K., Seino A. 1997; Intra- and intergeneric relationships of various actinomycete strains based on the acyl types of the muramyl residue in cell wall peptidoglycans examined in a glycolate test. Int J Syst Bacteriol 47:182–190 [CrossRef]
    [Google Scholar]
  29. Uchida K., Kudo T., Suzuki K., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [CrossRef]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  31. Zitouni A., Lamari L., Boudjella H., Badji B., Sabaou N., Gaouar A., Mathieu F., Lebrihi A., Labeda D. P. 2004; Saccharothrix algeriensis sp. nov., isolated from Saharan soil. Int J Syst Evol Microbiol 54:1377–1381 [CrossRef]
    [Google Scholar]
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