1887

Abstract

A Gram-stain-positive, rod-shaped, endospore-forming bacterium, strain CAU 9038, was isolated from a tidal-flat sediment of DaeYiJac Island, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach. The cell-wall peptidoglycan contained -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, the major isoprenoid quinone was MK-7 and the dominant cellular fatty acid was anteiso-C. The DNA G+C content was 51.6 mol%. 16S rRNA gene sequence analysis showed that the strain belonged to the genus , with <96.1 % sequence similarity to type strains of species with validly published names. The most closely related type strains to CAU 9038 were S3-4A (96.1 % similarity) and DSM 1355 (95.3 %). The phenotypic, chemotaxonomic and genotypic data clearly indicated that strain CAU 9038 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAU 9038 (=KCTC 13717 =CCUG 59129).

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2011-02-01
2024-04-20
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References

  1. Akaracharanya A., Lorliam W., Tanasupawat S., Lee K. C., Lee J. C. 2009; Paenibacillus cellulositrophicus sp. nov., a cellulolytic bacterium from Thai soil. Int J Syst Evol Microbiol 59:2680–2684 [CrossRef]
    [Google Scholar]
  2. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks, and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 64:253–260
    [Google Scholar]
  3. Ash C., Priest F. G., Collins M. D. 1994 Paenibacillus gen. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List no. 51. Int J Syst Bacteriol 44, 852 [CrossRef]
  4. Berge O., Guinebretiere M. H., Achouak W., Normand P., Heulin T. 2002; Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Microbiol 52:607–616
    [Google Scholar]
  5. Chou J. H., Chou Y. J., Lin K. Y., Sheu S. Y., Sheu D. S., Arun A. B., Young C. C., Chen W. M. 2007; Paenibacillus fonticola sp. nov., isolated from a warm spring. Int J Syst Evol Microbiol 57:1346–1350 [CrossRef]
    [Google Scholar]
  6. Chou J. H., Lee J. H., Lin M. C., Chang P. S., Arun A. B., Young C. C., Chen W.-M. 2009; Paenibacillus contaminans sp. nov., isolated from a contaminated laboratory plate. Int J Syst Evol Microbiol 59:125–129 [CrossRef]
    [Google Scholar]
  7. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  10. Felsenstein J. 1989; phylip – phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  11. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [CrossRef]
    [Google Scholar]
  12. Gordon R. E., Mihm J. M. 1962; Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 98:628–636
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp 21–132 Edited by Munro H. H. New York: Academic Press;
    [Google Scholar]
  14. Khianngam S., Tanasupawat S., Lee J. S., Lee K. C., Akaracharanya A. 2009a; Paenibacillus siamensis sp. nov., Paenibacillus septentrionalis sp. nov. and Paenibacillus montaniterrae sp. nov., xylanase-producing bacteria from Thai soils. Int J Syst Evol Microbiol 59:130–134 [CrossRef]
    [Google Scholar]
  15. Khianngam S., Akaracharanya A., Tanasupawat S., Lee K. C., Lee J. S. 2009b; Paenibacillus thailandensis sp. nov. and Paenibacillus nanensis sp. nov., xylanase-producing bacteria isolated from soil. Int J Syst Evol Microbiol 59:564–568 [CrossRef]
    [Google Scholar]
  16. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  17. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  18. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. other authors 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121 [CrossRef]
    [Google Scholar]
  19. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  20. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188:221–233 [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  22. Montes M. J., Mercade E., Bozal N., Guinea J. 2004; Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int J Syst Evol Microbiol 54:1521–1526 [CrossRef]
    [Google Scholar]
  23. Nam S. W., Kim W., Chun J., Goodfellow M. 2004; Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 54:1209–1212 [CrossRef]
    [Google Scholar]
  24. Nelson D. M., Glawe A. J., Labeda D. P., Cann I. K. O., Mackie R. I. 2009; Paenibacillus tundrae sp. nov. and Paenibacillus xylanexedens sp. nov., psychrotolerant, xylan-degrading bacteria from Alaskan tundra. Int J Syst Evol Microbiol 59:1708–1714 [CrossRef]
    [Google Scholar]
  25. Nicholson W. L., Setlow P. 1990; Sporulation, germination, and outgrowth. In Molecular Biological Methods for Bacillus pp 391–450 Edited by Harwood C. R., Cutting S. M. Chichester: Wiley;
    [Google Scholar]
  26. Roux V., Raoult D. 2004; Paenibacillus massiliensis sp. nov., Paenibacillus sanguinis sp. nov. and Paenibacillus timonensis sp. nov., isolated from blood cultures. Int J Syst Evol Microbiol 54:1049–1054 [CrossRef]
    [Google Scholar]
  27. Roux V., Fenner L., Raoult D. 2008; Paenibacillus provencensis sp. nov., isolated from human cerebrospinal fluid, and Paenibacillus urinalis sp. nov., isolated from human urine. Int J Syst Evol Microbiol 58:682–687 [CrossRef]
    [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  29. Schaeffer P., Millet J., Aubert J.-P. 1965; Catabolic repression of bacterial sporulation. Proc Natl Acad Sci U S A 54:704–711 [CrossRef]
    [Google Scholar]
  30. Scheldeman P., Goossens K., Rodriguez-Diaz M., Pil A., Goris J., Herman L., De Vos P., Logan N. A., Heyndrickx M. 2004; Paenibacillus lactis sp. nov., isolated from raw and heat-treated milk. Int J Syst Evol Microbiol 54:885–891 [CrossRef]
    [Google Scholar]
  31. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  32. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Emended description of Paenibacillus amylolyticus and description of Paenibacillus illinoisensis sp. nov. and Paenibacillus chibensis sp. nov. Int J Syst Bacteriol 47:299–306 [CrossRef]
    [Google Scholar]
  33. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Microbiology pp 409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  34. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology. pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  35. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R, Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  36. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  37. Tcherpakov M., Ben-Jacob E., Gutnick D. L. 1999; Paenibacillus dendritiformis sp. nov., proposal for a new pattern-forming species and its localization within a phylogenetic cluster. Int J Syst Bacteriol 49:239–246 [CrossRef]
    [Google Scholar]
  38. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  39. Uetanabaro A. P., Wahrenburg C., Hunger W., Pukall R., Spröer C., Stackebrandt E., de Canhos V. P., Claus D., Fritze D. 2003; Paenibacillus agarexedens sp. , nov., nom. rev., and Paenibacillus agaridevorans sp. nov. Int J Syst Evol Microbiol 531051–1057 [CrossRef]
    [Google Scholar]
  40. Vaz-Moreira I., Faria C., Nobre M. F., Schumann P., Nunes O. C., Manaia C. M. 2007; Paenibacillus humicus sp. nov., isolated from poultry litter compost. Int J Syst Evol Microbiol 57:2267–2271 [CrossRef]
    [Google Scholar]
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