1887

Abstract

A Gram-stain-negative, non-motile, non-spore-forming and short rod- or rod-shaped bacterial strain, designated 22-5, was isolated from a bluespotted cornetfish, , and subjected to taxonomic study. Strain 22-5 grew optimally at 30 °C and in the presence of 2–5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 22-5 belonged to the genus and joined the cluster comprising DD-R11 and ATCC 21588, with which strain 22-5 exhibited 97.4 and 96.9 % 16S rRNA gene sequence similarity, respectively. Strain 22-5 exhibited 94.0–96.6 % 16S rRNA gene sequence similarity with the other type strains of species of the genus . Strain 22-5 contained Q-10 as the predominant menaquinone and C 7 as the predominant fatty acid. In this study, . KCTC 22688 also contained Q-10 as the predominant isoprenoid quinone. The DNA G+C content of strain 22-5 was 63.6 mol%. Strain 22-5 exhibited 44 and 32 % DNA–DNA relatedness to . KACC 11518 and . KCTC 22688, respectively. On the basis of phenotypic, phylogenetic and genetic data, strain 22-5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 22-5 (=KCTC 22803 =CCUG 58401).

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2010-12-01
2020-09-22
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References

  1. Anzai Y., Kim H., Park J.-Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
    [Google Scholar]
  2. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium , Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes pp 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer-Verlag;
    [Google Scholar]
  3. Berry A., Janssens D., Hümbelin M., Jore J. P. M., Hoste B., Cleenwerck I., Vancanneyt M., Bretzel W., Mayer A. F. other authors 2003; Paracoccus zeaxanthinifaciens sp. nov., a zeaxanthin-producing bacterium. Int J Syst Evol Microbiol 53:231–238 [CrossRef]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  6. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49:25–68 [CrossRef]
    [Google Scholar]
  7. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  8. Davis D. H., Doudoroff M., Stanier R. Y., Mandel M. 1969; Proposal to reject the genus Hydrogenomonas : taxonomic implications. Int J Syst Bacteriol 19:375–390 [CrossRef]
    [Google Scholar]
  9. Euzéby J. P. 2009; List of bacterial names with standing in nomenclature: a folder available on the Internet. [Last full update 6 February 2009] http://www.bacterio.net
  10. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  11. Kelly D. P., Rainey F. A., Wood A. P. 2006; The genus Paracoccus . In The Prokaryotes, 3rd edn. vol 5 pp 232–249 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  12. Khan S. T., Takaichi S., Harayama S. 2008; Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay. Int J Syst Evol Microbiol 58:383–386 [CrossRef]
    [Google Scholar]
  13. Kim B.-Y., Weon H.-Y., Yoo S.-H., Kwon S.-W., Cho Y.-H., Stackebrandt E., Go S.-J. 2006; Paracoccus homiensis sp. nov., isolated from a sea-sand sample. Int J Syst Evol Microbiol 56:2387–2390 [CrossRef]
    [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  15. Lanyí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  16. Lee J. H., Kim Y. S., Choi T.-J., Lee W. J., Kim Y. T. 2004; Paracoccus haeundaensis sp. nov., a Gram-negative, halophilic, astaxanthin-producing bacterium. Int J Syst Evol Microbiol 54:1699–1702 [CrossRef]
    [Google Scholar]
  17. Lee K.-B., Liu C.-T., Anzai Y., Kim H., Aono T., Oyaizu H. 2005; The hierarchical system of the ‘ Alphaproteobacteria ’: description of Hyphomonadaceae fam.nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55:1907–1919 [CrossRef]
    [Google Scholar]
  18. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  19. Liu Z.-P., Wang B.-J., Liu X.-Y., Dai X., Liu Y.-H., Liu S.-J. 2008; Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969. Int J Syst Evol Microbiol 58:257–261 [CrossRef]
    [Google Scholar]
  20. Pukall R., Laroche M., Kroppenstedt R. M., Schumann P., Stackebrandt E., Ulber R. 2003; Paracoccus seriniphilus sp. nov., an l-serine-dehydratase-producing coccus isolated from the marine bryozoan Bugula plumose . Int J Syst Evol Microbiol 53:443–447 [CrossRef]
    [Google Scholar]
  21. Roh S. W., Nam Y.-D., Chang H.-W., Kim K.-H., Kim M.-S., Shin K.-S., Yoon J.-H., Oh H.-M., Bae J.-W. 2009; Paracoccus aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 59:790–794 [CrossRef]
    [Google Scholar]
  22. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  23. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  24. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  25. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  28. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  29. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
    [Google Scholar]
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