1887

Abstract

A Gram-stain-negative, non-motile, non-spore-forming and short rod- or rod-shaped bacterial strain, designated 22-5, was isolated from a bluespotted cornetfish, , and subjected to taxonomic study. Strain 22-5 grew optimally at 30 °C and in the presence of 2–5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 22-5 belonged to the genus and joined the cluster comprising DD-R11 and ATCC 21588, with which strain 22-5 exhibited 97.4 and 96.9 % 16S rRNA gene sequence similarity, respectively. Strain 22-5 exhibited 94.0–96.6 % 16S rRNA gene sequence similarity with the other type strains of species of the genus . Strain 22-5 contained Q-10 as the predominant menaquinone and C 7 as the predominant fatty acid. In this study, . KCTC 22688 also contained Q-10 as the predominant isoprenoid quinone. The DNA G+C content of strain 22-5 was 63.6 mol%. Strain 22-5 exhibited 44 and 32 % DNA–DNA relatedness to . KACC 11518 and . KCTC 22688, respectively. On the basis of phenotypic, phylogenetic and genetic data, strain 22-5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 22-5 (=KCTC 22803 =CCUG 58401).

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2010-12-01
2019-12-13
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References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Baumann, P. & Baumann, L. ( 1981; ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes, pp. 1302–1331. Edited by Starr, M. P., Stolp, H., Trüper, H. G., Balows, A. & Schlegel, H. G.. Berlin. : Springer-Verlag.
    [Google Scholar]
  3. Berry, A., Janssens, D., Hümbelin, M., Jore, J. P. M., Hoste, B., Cleenwerck, I., Vancanneyt, M., Bretzel, W., Mayer, A. F. & other authors ( 2003; ). Paracoccus zeaxanthinifaciens sp. nov., a zeaxanthin-producing bacterium. Int J Syst Evol Microbiol 53, 231–238.[CrossRef]
    [Google Scholar]
  4. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  5. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  6. Cohen-Bazire, G., Sistrom, W. R. & Stanier, R. Y. ( 1957; ). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49, 25–68.[CrossRef]
    [Google Scholar]
  7. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London. : Cambridge University Press.
    [Google Scholar]
  8. Davis, D. H., Doudoroff, M., Stanier, R. Y. & Mandel, M. ( 1969; ). Proposal to reject the genus Hydrogenomonas: taxonomic implications. Int J Syst Bacteriol 19, 375–390.[CrossRef]
    [Google Scholar]
  9. Euzéby, J. P. ( 2009; ). List of bacterial names with standing in nomenclature: a folder available on the Internet. [Last full update 6 February 2009]. http://www.bacterio.net.
  10. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  11. Kelly, D. P., Rainey, F. A. & Wood, A. P. ( 2006; ). The genus Paracoccus. In The Prokaryotes, 3rd edn, vol. 5, pp. 232–249. Edited by Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. & Stackebrandt, E.. New York. : Springer.
    [Google Scholar]
  12. Khan, S. T., Takaichi, S. & Harayama, S. ( 2008; ). Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay. Int J Syst Evol Microbiol 58, 383–386.[CrossRef]
    [Google Scholar]
  13. Kim, B.-Y., Weon, H.-Y., Yoo, S.-H., Kwon, S.-W., Cho, Y.-H., Stackebrandt, E. & Go, S.-J. ( 2006; ). Paracoccus homiensis sp. nov., isolated from a sea-sand sample. Int J Syst Evol Microbiol 56, 2387–2390.[CrossRef]
    [Google Scholar]
  14. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  15. Lanyí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  16. Lee, J. H., Kim, Y. S., Choi, T.-J., Lee, W. J. & Kim, Y. T. ( 2004; ). Paracoccus haeundaensis sp. nov., a Gram-negative, halophilic, astaxanthin-producing bacterium. Int J Syst Evol Microbiol 54, 1699–1702.[CrossRef]
    [Google Scholar]
  17. Lee, K.-B., Liu, C.-T., Anzai, Y., Kim, H., Aono, T. & Oyaizu, H. ( 2005; ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55, 1907–1919.[CrossRef]
    [Google Scholar]
  18. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  19. Liu, Z.-P., Wang, B.-J., Liu, X.-Y., Dai, X., Liu, Y.-H. & Liu, S.-J. ( 2008; ). Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969. Int J Syst Evol Microbiol 58, 257–261.[CrossRef]
    [Google Scholar]
  20. Pukall, R., Laroche, M., Kroppenstedt, R. M., Schumann, P., Stackebrandt, E. & Ulber, R. ( 2003; ). Paracoccus seriniphilus sp. nov., an l-serine-dehydratase-producing coccus isolated from the marine bryozoan Bugula plumose. Int J Syst Evol Microbiol 53, 443–447.[CrossRef]
    [Google Scholar]
  21. Roh, S. W., Nam, Y.-D., Chang, H.-W., Kim, K.-H., Kim, M.-S., Shin, K.-S., Yoon, J.-H., Oh, H.-M. & Bae, J.-W. ( 2009; ). Paracoccus aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 59, 790–794.[CrossRef]
    [Google Scholar]
  22. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  23. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  24. Staley, J. T. ( 1968; ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1942.
    [Google Scholar]
  25. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  27. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  28. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  29. Yoon, J.-H., Kim, I.-G., Shin, D.-Y., Kang, K. H. & Park, Y.-H. ( 2003; ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef]
    [Google Scholar]
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