1887

Abstract

A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8, was isolated from an acidic (pH 4.0–4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2–0.3 μm in diameter and 0.8–3.0 μm long) and irregular cocci (0.2–0.8 μm in diameter). The culture utilized H/CO to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8 is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, gen. nov., sp. nov. The type strain of is 6A8 (=DSM 21154 =JCM 14090).

Keyword(s): SSU, small subunit
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2011-01-01
2019-09-20
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