1887

Abstract

A thermophilic, Gram-positive bacterium that formed a branched vegetative mycelium was isolated from compost. The strain, designated I3, grew at temperatures between 35 and 62 °C, with optimum growth at 50–55 °C. No growth was observed below 29 °C or above 65 °C. The pH range for growth was 5.7–10.0, the pH for optimum growth was 7.0 and no growth was observed below pH 5.6 or above pH 10.8. The DNA G+C content of strain I3 was 69.2 mol%. The major fatty acids found were C iso (14.2 %), C anteiso (12.1 %), C iso (16.3 %) and C anteiso (21.7 %). The major menaquinones were MK-9(H), MK-10(H) and MK-11(H). The cell wall contained glutamic acid, glycine, alanine and -diaminopimelic acid in a molar ratio of 1.0 : 3.9 : 0.6 : 0.5. The polar lipids consisted of ninhydrin-positive phosphoglycolipids, phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. The cell-wall sugars were rhamnose and arabinose. 16S rRNA gene sequence analysis assigned this actinomycete to the family , but its 16S rRNA gene sequence shared no more than 95.5 % similarity with those of other members of the family. The chemotaxonomic and phenotypic characteristics of strain I3 differed in some respects from those of members of the genus , the most closely related genus. Therefore, strain I3 represents a novel species in a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is I3 (=JCM 16421=DSM 22891).

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2011-01-01
2019-10-23
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