1887

Abstract

A halophilic, filamentous actinomycete strain, designated YIM 93246, was isolated from a salt lake in Xinjiang province, north-west China, and subjected to polyphasic taxonomic characterization. The isolate grew in the presence of 7–23 % (w/v) NaCl, but not in the absence of NaCl. Strain YIM 93246 had particular morphological properties, forming aerial mycelium that had long spore chains and pseudosporangium-like, rhiziform spore aggregates at maturity. -DAP was the cell-wall diamino acid and glucosamine, mannose, glucose, arabinose and galactose were the cell-wall sugars. The major fatty acids were iso-C, anteiso-C and anteiso-C. MK-9 (H) was the predominant menaquinone and the genomic DNA G+C content was 70.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 93246 clustered with the genus . The sequence similarities between strain YIM 93246 and , and were 96.9, 96.9 and 96.6 %, respectively. Based on morphological, physiological and chemotaxonomic differences, and phylogenetic analysis, a novel genus and species, gen. nov., sp. nov., is proposed. The type strain of the species is YIM 93246 (=DSM 45211=KCTC 19409). Additionally, phylogenetic analysis placed the genus together with strain YIM 93246 within the order as an independent lineage, clearly distinguished from other described suborders of the class . Hence, based on phylogenetic characteristics, the genus together with the newly proposed genus are proposed to be classified as fam. nov. and subord. nov.

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2011-01-01
2019-12-15
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vol. , part 1, pp. 194-200

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. Polar lipid composition of strain YIM 93246 grown in GTY medium (15% NaCl, w/v). The polar lipids were identified as follows: DPG, diphosphatidylglycerol; PL, unknown phospholipid; PGL, unknown phosphoglycolipid; PI, phosphatidylinositol; PIM, phosphatidylinositol mannoside; GL, unknown glycolipid.

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