1887

Abstract

A halophilic, filamentous actinomycete strain, designated YIM 93246, was isolated from a salt lake in Xinjiang province, north-west China, and subjected to polyphasic taxonomic characterization. The isolate grew in the presence of 7–23 % (w/v) NaCl, but not in the absence of NaCl. Strain YIM 93246 had particular morphological properties, forming aerial mycelium that had long spore chains and pseudosporangium-like, rhiziform spore aggregates at maturity. -DAP was the cell-wall diamino acid and glucosamine, mannose, glucose, arabinose and galactose were the cell-wall sugars. The major fatty acids were iso-C, anteiso-C and anteiso-C. MK-9 (H) was the predominant menaquinone and the genomic DNA G+C content was 70.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 93246 clustered with the genus . The sequence similarities between strain YIM 93246 and , and were 96.9, 96.9 and 96.6 %, respectively. Based on morphological, physiological and chemotaxonomic differences, and phylogenetic analysis, a novel genus and species, gen. nov., sp. nov., is proposed. The type strain of the species is YIM 93246 (=DSM 45211=KCTC 19409). Additionally, phylogenetic analysis placed the genus together with strain YIM 93246 within the order as an independent lineage, clearly distinguished from other described suborders of the class . Hence, based on phylogenetic characteristics, the genus together with the newly proposed genus are proposed to be classified as fam. nov. and subord. nov.

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2011-01-01
2024-04-24
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References

  1. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  5. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  6. Kelly K. L. 1964 Inter-Society Color Council-National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Lee S. D. 2008; Jiangella alkaliphila sp. nov., an actinobacterium isolated from a cave. Int J Syst Evol Microbiol 58:1176–1179 [CrossRef]
    [Google Scholar]
  9. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [CrossRef]
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  13. Qin S., Zhao G. Z., Li J., Zhu W. Y., Xu L. H., Li W. J. 2009; Jiangella alba sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis . Int J Syst Evol Microbiol 59:2162–2165 [CrossRef]
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  15. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
    [Google Scholar]
  16. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  17. Song L., Li W.-J., Wang Q.-L., Chen G.-Z., Zhang Y.-S., Xu L.-H. 2005; Jiangella gansuensis gen. nov., sp. nov. a novel actinomycete from a desert soil in north-west China. Int J Syst Evol Microbiol 55:881–884 [CrossRef]
    [Google Scholar]
  18. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  20. Tang S. K., Tian X. P., Zhi X. Y., Cai M., Wu J. Y., Yang L. L., Xu L. H., Li W. J. 2008; Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae . Int J Syst Evol Microbiol 58:2075–2080 [CrossRef]
    [Google Scholar]
  21. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W.-J. 2009a; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . Int J Syst Evol Microbiol 59:2025–2031 [CrossRef]
    [Google Scholar]
  22. Tang S. K., Wang Y., Lou K., Mao P. H., Xu L. H., Jiang C. L., Kim C. J., Li W. J. 2009b; Kocuria halotolerans sp. nov., a novel actinobacterium isolated from a saline soil in China. Int J Syst Evol Microbiol 59:1316–1320 [CrossRef]
    [Google Scholar]
  23. Tang S. K., Zhi X. Y., Wang Y., Wu J. Y., Lee J. C., Kim C. J., Lou K., Xu L. H., Li W. J. 2010; Haloactinobacterium album gen. nov., sp. nov. a novel halophilic actinobacterium and proposal of Ruaniaceae fam. nov. Int J Syst Evol Microbiol 60:2113–2119 [CrossRef]
    [Google Scholar]
  24. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol 4 pp 2463–2468 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  25. Zhi X. Y., Li W. J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria , with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [CrossRef]
    [Google Scholar]
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