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Volume 61, Issue 6

sp. nov., a halotolerant bacterium isolated from a salt field Free

Abstract

A novel Gram-stain-positive, motile, strictly aerobic bacterium, designated YIM 93174, was isolated from a salt field in Korea. Cells of this strain were rod-shaped and formed pale tangerine colonies and grew at pH 6.0–8.0 (optimal growth at pH 7.0), at 15–45 °C (optimum 28–37 °C) and at salinities of 0–10 % (w/v) NaCl (optimum 0–2 % NaCl). Some phenotypic characters allowed differentiation of strain YIM 93174 from its nearest phylogenetic relatives. Comparative 16S rRNA gene sequence analysis showed that strain YIM 93174 belongs to the genus , exhibiting the highest level of sequence similarity with the type strain of (95.7 %), followed by those of s (94.9 %) and (94.5 %). The major fatty acids were iso-C, anteiso-C and iso-C. The cell-wall peptidoglycan was of the A1γ type, containing -diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 36.9 mol% and the predominant respiratory quinone was MK-7. The major polar lipids of strain YIM 93174 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unknown phospholipids. On the basis of the evidence from this polyphasic study, strain YIM 93174 represents a novel species of the genus , for which the name sp. nov. is proposed, with YIM 93174 ( = DSM 22388  = KCTC 13210  = CCTCC AA 208068) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833800)
  • 21C Frontier Program of Microbial Genomics and Applications
  • MEST, Korea
© 2011 IUMS
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2011-06-01
2024-04-25
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Bacillus luteolus sp. nov., a halotolerant bacterium isolated from a salt field
Int J Syst Evol Microbiol 61, 1344 (2011); https://doi.org/10.1099/ijs.0.021683-0
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