1887

Abstract

A novel Gram-stain-positive, motile, strictly aerobic bacterium, designated YIM 93174, was isolated from a salt field in Korea. Cells of this strain were rod-shaped and formed pale tangerine colonies and grew at pH 6.0–8.0 (optimal growth at pH 7.0), at 15–45 °C (optimum 28–37 °C) and at salinities of 0–10 % (w/v) NaCl (optimum 0–2 % NaCl). Some phenotypic characters allowed differentiation of strain YIM 93174 from its nearest phylogenetic relatives. Comparative 16S rRNA gene sequence analysis showed that strain YIM 93174 belongs to the genus , exhibiting the highest level of sequence similarity with the type strain of (95.7 %), followed by those of s (94.9 %) and (94.5 %). The major fatty acids were iso-C, anteiso-C and iso-C. The cell-wall peptidoglycan was of the A1γ type, containing -diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 36.9 mol% and the predominant respiratory quinone was MK-7. The major polar lipids of strain YIM 93174 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unknown phospholipids. On the basis of the evidence from this polyphasic study, strain YIM 93174 represents a novel species of the genus , for which the name sp. nov. is proposed, with YIM 93174 ( = DSM 22388  = KCTC 13210  = CCTCC AA 208068) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.021683-0
2011-06-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/6/1344.html?itemId=/content/journal/ijsem/10.1099/ijs.0.021683-0&mimeType=html&fmt=ahah

References

  1. Amoozegar M. A. , Sánchez-Porro C. , Rohban R. , Hajighasemi M. , Ventosa A. . ( 2009; ). Bacillus persepolensis sp. nov., a moderately halophilic bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 59:, 2352–2358. [CrossRef].[PubMed]
    [Google Scholar]
  2. Carrasco I. J. , Márquez M. C. , Yanfen X. , Ma Y. , Cowan D. A. , Jones B. E. , Grant W. D. , Ventosa A. . ( 2006; ). Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. . Int J Syst Evol Microbiol 56:, 599–604. [CrossRef].[PubMed]
    [Google Scholar]
  3. Cerny G. . ( 1978; ). Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5:, 113–122. [CrossRef]
    [Google Scholar]
  4. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  5. Cui X. L. , Mao P. H. , Zeng M. , Li W. J. , Zhang L. P. , Xu L. H. , Jiang C. L. . ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . . Int J Syst Evol Microbiol 51:, 357–363.[PubMed]
    [Google Scholar]
  6. DSMZ ( 2004; ). Key to murein (peptidoglycan) types. Braunschweig: Deutsche Sammlung von Mikroorganismen und Zellkulturen. http://www.dsmz.de/microorganisms/main.php?content_id=35
    [Google Scholar]
  7. Euzéby J. P. . ( 2010; ). Bacillus Cohn 1872, genus.. In List of Prokaryotic Names with Standing in Nomenclature. http://www.bacterio.cict.fr/b/bacillus.html
    [Google Scholar]
  8. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef].[PubMed]
    [Google Scholar]
  9. Felsenstein J. . ( 1985; ). Conference limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  11. Heyrman J. , Rodríguez-Díaz M. , Devos J. , Felske A. , Logan N. A. , De Vos P. . ( 2005; ). Bacillus arenosi sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. . Int J Syst Evol Microbiol 55:, 111–117. [CrossRef].[PubMed]
    [Google Scholar]
  12. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef].[PubMed]
    [Google Scholar]
  13. Kroppenstedt R. M. . ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  14. Lee J. C. , Lee G. S. , Park D. J. , Kim C. J. . ( 2008; ). Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil. . Int J Syst Evol Microbiol 58:, 2629–2634. [CrossRef].[PubMed]
    [Google Scholar]
  15. Leifson E. . ( 1960; ). Atlas of Bacterial Flagellation. London:: Academic Press;.
    [Google Scholar]
  16. Li W. J. , Xu P. , Schumann P. , Zhang Y.-Q. , Pukall R. , Xu L.-H. , Stackebrandt E. , Jiang C. L. . ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef].[PubMed]
    [Google Scholar]
  17. Logan N. A. , Berge O. , Bishop A. H. , Busse H. J. , De Vos P. , Fritze D. , Heyndrickx M. , Kämpfer P. , Rabinovitch L. et al. ( 2009; ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59:, 2114–2121. [CrossRef].[PubMed]
    [Google Scholar]
  18. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  19. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  20. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  21. Ruiz-García C. , Quesada E. , Martínez-Checa F. , Llamas I. , Urdaci M. C. , Béjar V. . ( 2005; ). Bacillus axarquiensis sp. nov. and Bacillus malacitensis sp. nov., isolated from river-mouth sediments in southern Spain. . Int J Syst Evol Microbiol 55:, 1279–1285. [CrossRef].[PubMed]
    [Google Scholar]
  22. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  24. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  25. Smibert R. M. , Krieg N. R. . ( 1981; ). General characterization. . In Manual of Methods for General Bacteriology, pp. 409–443. Edited by Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Philips G. B. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  26. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed]
    [Google Scholar]
  28. Tang S. K. , Wang Y. , Lee J. C. , Lou K. , Park D. J. , Kim C. J. , Li W. J. . ( 2010; ). Georgenia halophila sp. nov., a halophilic actinobacterium isolated from a salt lake. . Int J Syst Evol Microbiol 60:, 1317–1421. [CrossRef].[PubMed]
    [Google Scholar]
  29. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef].[PubMed]
    [Google Scholar]
  30. Vaishampayan P. , Miyashita M. , Ohnishi A. , Satomi M. , Rooney A. , La Duc M. T. , Venkateswaran K. . ( 2009; ). Description of Rummeliibacillus stabekisii gen. nov., sp. nov. and reclassification of Bacillus pycnus Nakamura et al. 2002 as Rummeliibacillus pycnus comb. nov.. Int J Syst Evol Microbiol 59:, 1094–1099. [CrossRef].[PubMed]
    [Google Scholar]
  31. Venkateswaran K. , Kempf M. , Chen F. , Satomi M. , Nicholson W. , Kern R. . ( 2003; ). Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant. . Int J Syst Evol Microbiol 53:, 165–172. [CrossRef].[PubMed]
    [Google Scholar]
  32. Williams S. T. . ( 1967; ). Sensitivity of streptomycetes to antibiotics as a taxonomic character. . J Gen Microbiol 46:, 151–160.[PubMed] [CrossRef]
    [Google Scholar]
  33. Xu P. , Li W. J. , Tang S. K. , Zhang Y. Q. , Chen G. Z. , Chen H. H. , Xu L. H. , Jiang C. L. . ( 2005; ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef].[PubMed]
    [Google Scholar]
  34. Yoon J.-H. , Oh T. K. . ( 2005; ). Bacillus litoralis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 55:, 1945–1948. [CrossRef].[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.021683-0
Loading
/content/journal/ijsem/10.1099/ijs.0.021683-0
Loading

Data & Media loading...

Supplements

Phylogenetic trees based on 16S rRNA gene sequence analysis, constructed using the maximum-parsimony (Fig. S1) and maximum-likelihood (Fig. S2) methods, showing the interrelationships of strain YIM 93174 and members of closely related species. [PDF of Supplementary Figs S1 and S2](54 KB)

PDF

Polar lipid composition of strain YIM 93174 grown in 5 % GTY medium. DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PIM, phosphatidylinositol mannoside; PL, unknown phospholipid.

IMAGE

Electron micrograph of rod-shaped cells of strain YIM 93174 , showing peritrichous flagellation.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error