1887

Abstract

A Gram-positive, aerobic, non-motile actinomycete, strain MN08-A0203, that formed pale yellow to orange-brown colonies and non-fragmented branched substrate mycelium is described. The strain, which produced very scanty aerial mycelium-like structures and scanty formation of spherical bodies on the aerial mycelium on Bennett’s agar medium, was studied in detail to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity studies, strain MN08-A0203 grouped with the genus , being most closely related to the type strain of (97.8 %). Chemotaxonomic data [menaquinone MK-9(H); iso-C (27 %), iso-C (18 %), C H (8 %), C 9-methyl (8 %) as major fatty acids; glucose, galactose, ribose, arabinose, mannose, rhamnose and xylose (trace) as whole cell sugars; diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids as polar phospholipids] supported allocation of the strain to the genus . Furthermore, the results of DNA–DNA hybridization of strain MN08-A0203 with the type strain of revealed that the two strains were genetically distinct from each other. Moreover, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain MN08-A0203 from closely related species. Thus, MN08-A0203 represents a novel species of the genus , for which the name sp. nov. is proposed, with MN08-A0203 ( = NBRC 105883  = VTCC D9-23) as the type strain.

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2011-05-01
2019-10-20
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vol. , part 5, pp. 1033 - 1038

Two dimentional TLC analysis of strains MN08-A0203 (a, b) and NBRC 105245 (b, c) showing the position of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), hydroxyphosphatidylethanolamine (OH-PE) and ninhydrin-positive phosphoglycolipids (NPG) as characteristic phospholipids. PME, phosphatidyl- -monomethylethanoloamine. Spray reagent used: ninhydrin (a, c) and Dittmer–Lestner (b, d) (Minnikin ., 1984).

An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. , 233–241.

DNA–DNA relatedness (%) of strain sp. nov. MN08-A0203 and the closely related type strain NBRC 105245

Data are the mean of five replications.



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