1887

Abstract

A novel strictly aerobic, orange-pigmented, Gram-stain-negative bacterium, designated strain GJ16, was isolated from coastal seawater of Gangjin Bay, the southernmost part of the Korean peninsula, and subjected to a polyphasic taxonomic study. It grew optimally at 25–30 °C, at pH 7.0–8.0 and in the presence of 3 % NaCl. Comparative 16S rRNA gene sequence analysis revealed that strain GJ16 formed a distinct lineage within the family and shared less than 91.2 % 16S rRNA gene sequence similarity with members of the genera , , , and . The predominant cellular fatty acids were iso-C (40.2 %), iso-C G (12.8 %), iso-C 3-OH (11.2 %) and C (6.6 %). The G+C content of the genomic DNA was 39.4 mol% and the major respiratory isoprenoid quinone was MK-6. On the basis of phenotypic and genotypic data, strain GJ16 represents a novel species in a new genus in the family , for which the name gen. nov., sp. nov. is proposed; the type strain is GJ16 (=KCTC 22649 =JCM 16082).

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2011-02-01
2019-10-14
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References

  1. Baumann, P. & Baumann, L. ( 1981; ). The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes, vol. 1, pp. 1302–1331. Edited by Starr, M. P., Stolp, H., Trüper, H. G., Balows, A. & Schlegel, H.. Berlin. : Springer.
    [Google Scholar]
  2. Bernardet, J.-F. & Nakagawa, Y. ( 2006; ). An introduction to the family Flavobacteriaceae. In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 455–480. Edited by Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. & Stackebrandt, E.. New York. : Springer.
    [Google Scholar]
  3. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  4. Bowman, J. P. ( 2006; ). The marine clade of the family Flavobacteriaceae: the genera Aequorivita, Arenibacter, Cellulophaga, Croceibacter, Formosa, Gelidibacter, Gillisia, Maribacter, Mesonia, Muricauda, Polaribacter, Psychroflexus, Psychroserpens, Robiginitalea, Salegentibacter, Tenacibaculum, Ulvibacter, Vitellibacter and Zobellia. In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 677–694. Edited by Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. & Stackebrandt, E.. New York. : Springer.
    [Google Scholar]
  5. Chun, J., Lee, J. H., Jung, Y. Y., Kim, M. J., Kim, S. I., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  6. CLSI ( 2003; ). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, 2nd edn. Approved standard M31-A2. Wayne, PA: Clinical and Laboratory Standards Institute.
  7. Cole, J. R., Chai, B., Marsh, T. L., Farris, R. J., Wang, Q., Kulam, S. A., Chandra, S., McGarrell, D. M., Schmidt, T. M. & other authors ( 2003; ). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1985; ). Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. Khan, S. T., Nakagawa, Y. & Harayama, S. ( 2006; ). Krokinobacter gen. nov., with three novel species, in the family Flavobacteriaceae. Int J Syst Evol Microbiol 56, 323–328.[CrossRef]
    [Google Scholar]
  10. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge. : Cambridge University Press.
    [Google Scholar]
  11. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  12. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt, E. & Goodfellow, M.. Chichester. : Wiley.
    [Google Scholar]
  13. Lau, S. C. K., Tsoi, M. M. Y., Li, X., Plakhotnikova, I., Dobretsov, S., Lau, K. W. K., Wu, M., Wong, P.-K., Pawlik, J. P. & Qian, P.-Y. ( 2005; ). Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. Int J Syst Evol Microbiol 55, 1589–1592.[CrossRef]
    [Google Scholar]
  14. Liu, Z.-P., Wang, B.-J., Dai, X., Liu, X.-Y. & Liu, S.-J. ( 2006; ). Zhouia amylolytica gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from sediment of the South China Sea. Int J Syst Evol Microbiol 56, 2825–2829.[CrossRef]
    [Google Scholar]
  15. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  16. Mitra, S., Matsuo, Y., Haga, T., Yasumoto-Hirose, M., Yoon, J., Kasai, H. & Yokota, A. ( 2009; ). Leptobacterium flavescens gen. nov., sp. nov., a marine member of the family Flavobacteriaceae, isolated from marine sponge and seawater. Int J Syst Evol Microbiol 59, 207–212.[CrossRef]
    [Google Scholar]
  17. Norris, J. R., Ribbons, D. W. & Varma, A. K. (editors)) ( 1985; ). Methods in Microbiology, vol. 18. London. : Academic Press.
    [Google Scholar]
  18. Schmidt, K., Connor, A. & Britton, G. ( 1994; ). Analysis of pigments: carotenoids and related polyenes. In Chemical Methods in Prokaryotic Systematics, pp. 403–461. Edited by Goodfellow, M. & O'Donnell, A. G.. Chichester. : Wiley.
    [Google Scholar]
  19. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characteristics. In Methods for General and Molecular Biology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  20. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. Tindall, B. J., Sikorski, J., Smibert, R. A. & Kreig, N. R. ( 2007; ). Phenotypic characterization and principles of comparative systematics. In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy, C. A., Beveridge, T. J., Breznak, J. A., Marzluf, G. A., Schmidt, T. M. & Snyder, L. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  23. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  24. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K. ( 2005; ). Dokdonia donghaensis gen. nov., sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55, 2323–2328.[CrossRef]
    [Google Scholar]
  25. ZoBell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
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vol. , part 2, pp. 325 - 329

Scanning electron micrograph of cells of gen. nov., sp. nov., strain GJ16 , grown on MA at 30 °C for 3 days. Bar, 2 µm.



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