1887

Abstract

A Gram-positive, aerobic to microaerophilic, non-motile bacterial strain, designated MJ21, was isolated from farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain MJ21 was placed within the genus , and exhibited relatively high levels of similarity to XIL01 (97.8 %), YIM 21741 (97.1 %), JCM 3346 (96.7 %), JCM 1376 (99.1 %), JCM 9632 (99.1 %), JCM 9633 (98.9 %) and JCM 9631 (96.5 %). Chemotaxonomic data also supported the classification of strain MJ21 within the genus . The new isolate contained MK-12 as the predominant menaquinone and rhamnose, galactose and xylose as cell-wall sugars. The major cellular fatty acids (>10 % of the total) were anteiso-C, anteiso-C and iso-C. Cell-wall amino acids were 2,4-diaminobutyric acid, glutamic acid, glycine and alanine. Diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and one unidentified phospholipid were detected as polar lipids. The DNA G+C content of strain MJ21 was 73.4 mol%. However, levels of DNA–DNA relatedness between strain MJ21 and the seven phylogenetically closest strains ranged from 14 to 56 %, showing clearly that the new isolate represents a novel genomic species. Strain MJ21 could be differentiated clearly from its phylogenetic neighbours on the basis of phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ21 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MJ21 ( = KCTC 19549  = JCM 16247).

Funding
This study was supported by the:
  • Eco-Technopia 21 Project, Ministry of Environment, Republic of Korea (Award 071-081-133)
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2011-06-01
2022-01-19
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