1887

Abstract

An actinobacterial strain, designated 72, was isolated from a traditional salt-fermented seafood in Korea. Colonies were coral red and cells were Gram-reaction-positive, non-motile rods. Strain 72 grew with 0–10 % (w/v) NaCl, at pH 7–10 and at 15–37 °C. Optimum growth conditions were 2 % NaCl, pH 7.0 and 30 °C. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain 72 belonged to the genus . The major cellular fatty acids (>5 %) were C, summed feature 3 (comprising Cω6 and/or Cω7), 10-methyl C, C, C and Cω9. 16S rRNA gene sequence analysis and DNA–DNA hybridization, coupled with physiological and biochemical tests, revealed genotypic and phenotypic differences between strain 72 and other members of the genus . Based on these data, strain 72 represents a novel species, for which the name sp. nov. is proposed. The type strain is 72 ( = JCM 16360  = KACC 21126).

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2011-09-01
2019-08-24
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Two-dimensional TLC of polar lipids of sp. nov. 72 , showing aminolipids stained with ninhydrin (a), phospholipids with Zinzadze reagent (b), glycolipids with α-naphthol (c) and total lipids with 5 % ethanolic molybdatophosphoric acid (d). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol; PIM, phosphatidylinositol mannoside; AL, unknown aminolipid; GL, unknown glycolipid; L, unknown lipid; PL, unknown phospholipid.

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One-dimensional TLC of mycolic acids of sp. nov. 72 and related members of the genus . Lanes: 1, JCM 13663 ; 2, DSM 45139 ; 3, JCM 6166 ; 4, sp. nov. 72 .

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