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Abstract

A rod-shaped, endospore-forming, Gram-reaction-variable bacterial strain, designated WPCB173, was isolated from freshwater collected from the Woopo wetland, Republic of Korea. Based on its phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analysis, the isolate was identified as being a member of the genus . Major polar lipids present in strain WPCB173 included phosphatidylethanolamine and several unidentified phospholipids. The diamino acid found in the cell-wall peptidoglycan was diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acid was anteiso-C (65.2 %). The DNA G+C content was 48.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WPCB173 clustered with species of the genus , its closest neighbours being WN9 (96.7 %) and DCY03 (96.4 %). DNA–DNA hybridization of strain WPCB173 with DCY03 and WN9 showed relatedness values of only 10 and 19 %, respectively. On the basis of the phenotypic and phylogenetic evidence, strain WPCB173 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the novel species is WPCB173 (=KCTC 13282 =JCM 16352).

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2011-03-01
2019-10-24
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vol. , part 3, pp. 529 - 534

Micrographs of cells of strain WPCB173 .

Polar lipid profile of strain WPCB173 after two-dimentional TLC.

Extended phylogenetic tree of the genus based on 16S rRNA gene sequences and reconstructed using the neighbor-joining method.

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