1887

Abstract

A group of 16 isolates with genotypic characteristics different from those of known species of the complex were cultured from ear biopsies of the rodents and trapped at five localities in South Carolina, USA, and from the tick feeding on . Multilocus sequence analysis of members of the novel species, involving the 16S rRNA gene, the 5S–23S () intergenic spacer region and the flagellin, and genes, was conducted and published previously and was used to clarify the taxonomic status of the novel group of isolates. Phylogenetic analysis based on concatenated sequences of the five analysed genomic loci showed that the 16 isolates clustered together but separately from other species in the complex. The analysed group therefore represents a novel species, formally described here as sp. nov., with the type strain SCW-22 (=ATCC BAA-1773 =DSM 22119).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.021436-0
2011-02-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/2/381.html?itemId=/content/journal/ijsem/10.1099/ijs.0.021436-0&mimeType=html&fmt=ahah

References

  1. Baranton, G., Postic, D., Saint Girons, I., Boerlin, P., Piffaretti, J.-C., Assous, M. & Grimont, P. A. D. ( 1992; ). Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int J Syst Bacteriol 42, 378–383.[CrossRef]
    [Google Scholar]
  2. Barbour, A. G. & Hayes, S. F. ( 1986; ). Biology of Borrelia species. Microbiol Rev 50, 381–400.
    [Google Scholar]
  3. Burgdorfer, W., Barbour, A. G., Hayes, S. F., Benach, J. L., Grunwaldt, E. & Davis, J. P. ( 1982; ). Lyme disease – a tick-borne spirochetosis? Science 216, 1317–1319.[CrossRef]
    [Google Scholar]
  4. Canica, M. M., Nato, F., du Merle, L., Mazie, J. C., Baranton, G. & Postic, D. ( 1993; ). Monoclonal antibodies for identification of Borrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis. Scand J Infect Dis 25, 441–448.[CrossRef]
    [Google Scholar]
  5. Chu, C.-Y., Liu, W., Jiang, B.-G., Wang, D.-M., Jiang, W.-J., Zhao, Q.-M., Zhang, P.-H., Wang, Z.-X., Tang, G.-P. & other authors ( 2008; ). Novel genospecies of Borrelia burgdorferi sensu lato from rodents and ticks in southwestern China. J Clin Microbiol 46, 3130–3133.[CrossRef]
    [Google Scholar]
  6. Clark, K., Hendricks, A. & Burge, D. ( 2005; ). Molecular identification and analysis of Borrelia burgdorferi sensu lato in lizards in the southeastern United States. Appl Environ Microbiol 71, 2616–2625.[CrossRef]
    [Google Scholar]
  7. Fukunaga, M., Hamase, A., Okada, K. & Nakao, M. ( 1996a; ). Borrelia tanukii sp. nov. and Borrelia turdae sp. nov. found from ixodid ticks in Japan: rapid species identification by 16S rRNA gene-targeted PCR analysis. Microbiol Immunol 40, 877–881.[CrossRef]
    [Google Scholar]
  8. Fukunaga, M., Okada, K., Nakao, M., Konishi, T. & Sato, Y. ( 1996b; ). Phylogenetic analysis of Borrelia species based on flagellin gene sequences and its application for molecular typing of Lyme disease borreliae. Int J Syst Bacteriol 46, 898–905.[CrossRef]
    [Google Scholar]
  9. Guilliams, B. & Francl, K. ( 2008; ). Neotoma floridana – eastern woodrat. In Animal Diversity Web. Ann Arbor, MI: University of Michigan Museum of Zoology. http://animaldiversity.ummz.umich.edu/site/accounts/information/Neotoma_floridana.html.
  10. Guindon, S. & Gascuel, O. ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52, 696–704.[CrossRef]
    [Google Scholar]
  11. Güner, E. S., Hashimoto, N., Takada, N., Kaneda, K., Imai, Y. & Masuzawa, T. ( 2003; ). First isolation and characterization of Borrelia burgdorferi sensu lato strains from Ixodes ricinus ticks in Turkey. J Med Microbiol 52, 807–813.[CrossRef]
    [Google Scholar]
  12. Guy, E. C. & Stanek, G. ( 1991; ). Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction. J Clin Pathol 44, 610–611.[CrossRef]
    [Google Scholar]
  13. Johnson, R. C., Schmidt, G. P., Hyde, F. W., Steigerwalt, A. G. & Brenner, D. J. ( 1984; ). Borrelia burgdorferi sp. nov.: etiologic agent of Lyme disease. Int J Syst Bacteriol 34, 496–497.[CrossRef]
    [Google Scholar]
  14. Kawabata, H., Masuzawa, T. & Yanagihara, Y. ( 1993; ). Genomic analysis of Borrelia japonica sp. nov. isolated from Ixodes ovatus in Japan. Microbiol Immunol 37, 843–848.[CrossRef]
    [Google Scholar]
  15. Le Fleche, A., Postic, D., Girardet, K., Peter, O. & Baranton, G. ( 1997; ). Characterization of Borrelia lusitaniae sp. nov. by 16S ribosomal DNA sequence analysis. Int J Syst Bacteriol 47, 921–925.[CrossRef]
    [Google Scholar]
  16. Marconi, R. T., Lubke, L., Hauglum, W. & Garon, C. F. ( 1992; ). Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes. J Clin Microbiol 30, 628–632.
    [Google Scholar]
  17. Marconi, R. T., Liveris, D. & Schwartz, I. ( 1995; ). Identification of novel insertion elements, restriction fragment length polymorphism patterns, and discontinuous 23S rRNA in Lyme disease spirochetes: phylogenetic analyses of rRNA genes and their intergenic spacers in Borrelia japonica sp. nov. and genomic group 21038 (Borrelia andersonii sp. nov.) isolates. J Clin Microbiol 33, 2427–2434.
    [Google Scholar]
  18. Margos, G., Vollmer, S. A., Cornet, M., Garnier, M., Fingerle, V., Wilske, B., Bormane, A., Vitorino, L., Collares-Pereira, M. & other authors ( 2009; ). A new Borrelia species defined by multilocus sequence analysis of housekeeping genes. Appl Environ Microbiol 75, 5410–5416.[CrossRef]
    [Google Scholar]
  19. Masuzawa, T., Takada, N., Kudeken, M., Fukui, T., Yano, Y., Ishiguro, F., Kawamura, Y., Imai, Y. & Ezaki, T. ( 2001; ). Borrelia sinica sp. nov., a Lyme disease-related Borrelia species isolated in China. Int J Syst Evol Microbiol 51, 1817–1824.[CrossRef]
    [Google Scholar]
  20. Oliver, J. H., Jr ( 1996; ). Lyme borreliosis in the southern United States: a review. J Parasitol 82, 926–935.[CrossRef]
    [Google Scholar]
  21. Oliver, J. H., Jr, Clark, K. L., Chandler, F. W., Jr, Tao, L., James, A. M., Banks, C. W., Huey, L. O., Banks, A. R., Williams, D. C. & Durden, L. A. ( 2000; ). Isolation, cultivation, and characterization of Borrelia burgdorferi from rodents and ticks in the Charleston area of South Carolina. J Clin Microbiol 38, 120–124.
    [Google Scholar]
  22. Posada, D. & Crandall, K. A. ( 1998; ). ModelTest: testing the model of DNA substitution. Bioinformatics 14, 817–818.[CrossRef]
    [Google Scholar]
  23. Postic, D., Assous, M. V., Grimont, P. A. D. & Baranton, G. ( 1994; ). Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol 44, 743–752.[CrossRef]
    [Google Scholar]
  24. Postic, D., Ras, N. M., Lane, R. S., Hendson, M. & Baranton, G. ( 1998; ). Expanded diversity among Californian Borrelia isolates and description of Borrelia bissettii sp. nov. (formerly Borrelia group DN127). J Clin Microbiol 36, 3497–3504.
    [Google Scholar]
  25. Postic, D., Garnier, M. & Baranton, G. ( 2007; ). Multilocus sequence analysis of atypical Borrelia burgdorferi sensu lato isolates – description of Borrelia californiensis sp. nov., and genomospecies 1 and 2. Int J Med Microbiol 297, 263–271.[CrossRef]
    [Google Scholar]
  26. Richter, D., Postic, D., Sertour, N., Livey, I., Matuschka, F.-R. & Baranton, G. ( 2006; ). Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov. Int J Syst Evol Microbiol 56, 873–881.[CrossRef]
    [Google Scholar]
  27. Rudenko, N., Golovchenko, M., Grubhoffer, L. & Oliver, J. H., Jr ( 2009a; ). Borrelia carolinensis sp. nov., a new (14th) member of the Borrelia burgdorferi sensu lato complex from the southeastern region of the United States. J Clin Microbiol 47, 134–141.[CrossRef]
    [Google Scholar]
  28. Rudenko, N., Golovchenko, M., Lin, T., Gao, L., Grubhoffer, L. & Oliver, J. H., Jr ( 2009b; ). Delineation of a new species of the Borrelia burgdorferi sensu lato complex, Borrelia americana sp. nov. J Clin Microbiol 47, 3875–3880.[CrossRef]
    [Google Scholar]
  29. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  30. Wang, G., van Dam, A. P., Le Fleche, A., Postic, D., Peter, O., Baranton, G., de Boer, R., Spanjaard, L. & Dankert, J. ( 1997; ). Genetic and phenotypic analysis of Borrelia valaisiana sp. nov. (Borrelia genomic groups VS116 and M19). Int J Syst Bacteriol 47, 926–932.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.021436-0
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error