1887

Abstract

Strain M1-8 was isolated from jeotgal, a Korean salt-fermented food. Cells were aerobic, non-motile, Gram-reaction-positive and rod-shaped. Colonies were cream-coloured and circular with entire margins. Strain M1-8 exhibited optimal growth at 25–30 °C and pH 7.0–8.0 and in 0–4 % (w/v) NaCl. The strain tolerated up to 10.0 mM Cr(VI). Phylogenetic analyses of 16S rRNA gene sequences indicated that strain M1-8 represents a novel species in the genus . The 16S rRNA gene sequence of M1-8 exhibited 98.1 % similarity to that of subsp. L-1. The new isolate was clustered with species on a 16S rRNA gene sequence-based phylogenetic tree. The chromosomal DNA G+C content of strain M1-8 was 62.8 mol%. Its cell-wall peptidoglycan contained 2,4-diaminobutyric acid, glutamic acid, alanine, glycine and -aminobutyric acid. The major menaquinone was MK-11 and the predominant fatty acids were anteiso-C (63.6 %), anteiso-C (16.7 %) and iso-C (14.2 %). The polar lipid profile of strain M1-8 contained diphosphatidylglycerol and one unknown glycolipid. Significant genotypic and phenotypic differences were found between strain M1-8 and other species. These differentiating characteristics indicate that strain M1-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M1-8 (=KACC 21127 =JCM 16362).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.021360-0
2011-03-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/3/502.html?itemId=/content/journal/ijsem/10.1099/ijs.0.021360-0&mimeType=html&fmt=ahah

References

  1. Behrendt, U., Ulrich, A. & Schumann, P. ( 2008; ). Leucobacter tardus sp. nov., isolated from the phyllosphere of Solanum tuberosum L. Int J Syst Evol Microbiol 58, 2574–2578.[CrossRef]
    [Google Scholar]
  2. Bousfield, I. J., Keddie, R. M., Dando, T. R. & Shaw, S. ( 1985; ). Simple rapid methods of cell wall analysis as an aid in the identification of aerobic coryneform bacteria. In Chemical Methods in Bacterial Systematics, pp. 221–236. Edited by Goodfellow, M. & Minnikin, D. E.. London. : Academic Press.
    [Google Scholar]
  3. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  4. Chun, J., Lee, J. H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  6. Gonzalez, J. M. & Saiz-Jimenez, C. ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4, 770–773.[CrossRef]
    [Google Scholar]
  7. Halpern, M., Shaked, T., Pukall, R. & Schumann, P. ( 2009; ). Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass. Int J Syst Evol Microbiol 59, 665–670.[CrossRef]
    [Google Scholar]
  8. Hirayama, H., Tamaoka, J. & Horikoshi, K. ( 1996; ). Improved immobilization of DNA to microwell plates for DNA-DNA hybridization. Nucleic Acids Res 24, 4098–4099.[CrossRef]
    [Google Scholar]
  9. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  10. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  11. Lin, Y. C., Uemori, K., de Briel, D. A., Arunpairojana, V. & Yokota, A. ( 2004; ). Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. Int J Syst Evol Microbiol 54, 1669–1676.[CrossRef]
    [Google Scholar]
  12. Morais, P. V., Francisco, R., Branco, R., Chung, A. P. & da Costa, M. S. ( 2004; ). Leucobacter chromiireducens sp. nov., and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment. Syst Appl Microbiol 27, 646–652.[CrossRef]
    [Google Scholar]
  13. Morais, P. V., Paulo, C., Francisco, R., Branco, R., Chung, A. P. & da Costa, M. S. ( 2006; ). Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress. Syst Appl Microbiol 29, 414–421.[CrossRef]
    [Google Scholar]
  14. Muir, R. E. & Tan, M. W. ( 2007; ). Leucobacter chromiireducens subsp. solipictus subsp. nov., a pigmented bacterium isolated from the nematode Caenorhabditis elegans, and emended description of L. chromiireducens. Int J Syst Evol Microbiol 57, 2770–2776.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Somvanshi, V. S., Lang, E., Schumann, P., Pukall, R., Kroppenstedt, R. M., Ganguly, S. & Stackebrandt, E. ( 2007; ). Leucobacter iarius sp. nov., in the family Microbacteriaceae. Int J Syst Evol Microbiol 57, 682–686.[CrossRef]
    [Google Scholar]
  17. Suh, H. K. & Yoon, S. S. ( 1987; ). A study on the regional characteristics of Korean chotkal. Korean J Dietary Cult 2, 45–54.
    [Google Scholar]
  18. Takeuchi, M., Weiss, N., Schumann, P. & Yokota, A. ( 1996; ). Leucobacter komagatae gen. nov., sp. nov., a new aerobic gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 967–971.[CrossRef]
    [Google Scholar]
  19. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  21. Tindall, B. J. ( 1990; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  22. Tittsler, R. P. & Sandholzer, L. A. ( 1936; ). The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31, 575–580.
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. Xin, H., Itoh, T., Zhou, P., Suzuki, K., Kamekura, M. & Nakase, T. ( 2000; ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50, 1297–1303.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.021360-0
Loading
/content/journal/ijsem/10.1099/ijs.0.021360-0
Loading

Data & Media loading...

Supplements

One-dimensional TLC of peptidoglycan hydrolysates showing the amino acid compositions of strain M1-8 (lane 1) and JCM 9414 (lane 2). DAB, 2,4-Diaminobutyric acid; GABA, γ-aminobutyric acid; Ala, alanine; Glu, glutamic acid; Gly, glycine; Thr, threonine.

IMAGE

One-dimensional TLC of polar lipids from subsp. JCM 15573 (lane 1), strain M1-8 (lane 2) and JCM 9414 (lane 3). Phospholipids and glycolipids were respectively detected with Zinzadze reagent (a) and α-naphthol reagent (b). PG, Phosphatidylglycerol; DPG, diphosphatidylglycerol; GL, glycolipid.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error