1887

Abstract

In the context of studying the effects of transgenic fructan-producing potatoes on the community structure of phyllosphere bacteria, a group of strains closely related to the species was isolated. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the new isolates and DSM 19088 formed a lineage at the genus level and this finding was supported by chemotaxonomic characterization. The peptidoglycan type of the representative isolate, K134/01, and DSM 19088 was B2γ, with - and -diaminobutyric acid as the diagnostic diamino acid and glycine, alanine and -3-hydroxyglutamic acid. The almost-complete substitution of glutamic acid by -3-hydroxyglutamic acid supported the differentiation of the new strains from recognized species of the genus . Furthermore, the detection of substantial amounts of the fatty acid cyclohexyl-C in the new isolates and DSM 19088 was a prominent chemotaxonomic feature for a clear demarcation of these strains from all genera of the family that display the B2γ cell-wall type. Comparative phylogenetic and phenotypic analyses of the isolates and DSM 19088 revealed the separate species status of the isolates. On the basis of these results, it is proposed that should be classified as the type species of a novel genus, gen. nov., with the name gen. nov., comb. nov. (type strain wged11 = CGMCC 4.3491 = JCM 13908 = DSM 19088 = NBRC 104580). The phyllosphere isolates are assigned to a novel species, sp. nov. (type strain K134/01 = DSM 19813 = LMG 24387 = NBRC 106740).

Funding
This study was supported by the:
  • Federal Ministry of Education and Research (Award no. 0312632D)
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2011-05-01
2022-05-19
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