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Abstract

A Gram-negative bacterium, designated KIS13-15, was isolated from soil in Korea. Cells were strictly aerobic rods and were motile with 1–3 subpolar flagella. 16S rRNA gene sequence analysis revealed that strain KIS13-15 belonged to the order of the class and was closely related to GR15-1 (97.9 % 16S rRNA gene sequence similarity) and Soe1 (96.8 %). Strain KIS13-15 exhibited 38 and 29 % DNA–DNA relatedness with KACC 11703 and DSM 11499, respectively. The major fatty acids (>10 % of the total) were Cω7 (40.6 %) and C (12.4 %). The major isoprenoid quinone was ubiquinone 8. DNA G+C content was 67.3 mol%. The phenotypic characterization combined with 16S rRNA gene sequence analysis and DNA–DNA hybridization clearly classified strain KIS13-15 in a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KIS13-15 ( = KACC 12736  = JCM 15425).

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2011-05-01
2019-12-05
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. (editors) ( 1987;). Current Protocols in Molecular Biology. New York:: Wiley;.
    [Google Scholar]
  2. Breznak J. A., Costilow R. N.. ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  4. Famintzin A.. ( 1892;). Eine neue Bacterienform: Nevskia ramosa. . Bull Acad Imp Sci St Petersbourg New Ser 2 34:, 481–486 (in German).
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  7. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A.. ( 1996;). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 234–239. [CrossRef][PubMed]
    [Google Scholar]
  8. Kämpfer P., Steiof M., Dott W.. ( 1991;). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21:, 227–251. [CrossRef]
    [Google Scholar]
  9. Kimura M.. ( 1983;). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M.. ( 2004;). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef][PubMed]
    [Google Scholar]
  11. Kwon S. W., Kim J. S., Park I. C., Yoon S. H., Park D. H., Lim C. K., Go S. J.. ( 2003;). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. . Int J Syst Evol Microbiol 53:, 21–27. [CrossRef][PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid-chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  13. Reasoner D. J., Geldreich E. E.. ( 1985;). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49:, 1–7.[PubMed]
    [Google Scholar]
  14. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  15. Seldin L., Dubnau D.. ( 1985;). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35:, 151–154. [CrossRef]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Stürmeyer H., Overmann J., Babenzien H. D., Cypionka H.. ( 1998;). Ecophysiological and phylogenetic studies of Nevskia ramosa in pure culture. . Appl Environ Microbiol 64:, 1890–1894.[PubMed]
    [Google Scholar]
  18. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  20. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  21. Weon H.-Y., Kim B.-Y., Son J.-A., Song M.-H., Kwon S.-W., Go S.-J., Stackebrandt E.. ( 2008;). Nevskia soli sp. nov., isolated from soil cultivated with Korean ginseng. . Int J Syst Evol Microbiol 58:, 578–580. [CrossRef][PubMed]
    [Google Scholar]
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vol. , part 5, pp. 1226 - 1229

Transmission electron micrograph of a cell of strain KIS13-15 after growth for 8 days on R2A agar. Bar, 1 µm.



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