1887

Abstract

Two non-motile, orange- or yellow-pigmented bacteria, designated strains KYW48 and KYW147, were isolated from seawater collected from the South Sea, Republic of Korea. Cells of both strains were Gram-reaction-negative, aerobic and catalase- and oxidase-positive. The major fatty acids of strain KYW48 were Cω7 (35.3 %), summed feature 3 (iso-C 2-OH and/or Cω7) (22.7 %), Cω6 (19.8 %), C 2-OH (7.4 %) and C (5.9 %), and those of strain KYW147 were Cω7 (36.0 %), summed feature 3 (18.3 %), C (14.7 %), 11-methyl Cω7 (10.7 %), C 2-OH (9.1 %) and Cω9 (8.0 %). The predominant isoprenoid quinone of both strains was ubiquinone 10 (Q-10). The DNA G+C contents of strains KYW48 and KYW147 were 63.8 and 67.2 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequences showed that strains KYW48 and KYW147 were grouped with the members of the family and formed a distinct clade with the members of the genus (<95.7 % sequence similarity). On the basis of the evidence presented in this study, the novel species sp. nov. (type strain KYW48  = KCTC 22736  = JCM 16345) and sp. nov. (type strain KYW147  = KCTC 22735  = JCM 16339) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.021196-0
2011-04-01
2019-12-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/709.html?itemId=/content/journal/ijsem/10.1099/ijs.0.021196-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F. , Nakagawa Y. , Holmes B. . ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070 [CrossRef] [PubMed].
    [Google Scholar]
  3. Chun J. , Goodfellow M. . ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45:, 240–245. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261 [CrossRef] [PubMed].
    [Google Scholar]
  5. CLSI (2003). Performance standards for antimicrobial disk susceptibility tests, 8th edn. Approved Standard M2-A8. Wayne, PA: Clinical and Laboratory Standards Institute.
  6. Collins M. D. . ( 1985; ). Analysis of isoprenoid quinones. . Methods Microbiol 18:, 329–366. [CrossRef]
    [Google Scholar]
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Felsenstein, J. (1993). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  10. Gordon R. E. , Mihm J. M. . ( 1962; ). Identification of Nocardia caviae (Erikson) nov. comb.. Ann N Y Acad Sci 98:, 628–636. [CrossRef]
    [Google Scholar]
  11. Ivanova E. P. , Bowman J. P. , Lysenko A. M. , Zhukova N. V. , Gorshkova N. M. , Kuznetsova T. A. , Kalinovskaya N. I. , Shevchenko L. S. , Mikhailov V. V. . ( 2005; ). Erythrobacter vulgaris sp. nov., a novel organism isolated from the marine invertebrates. . Syst Appl Microbiol 28:, 123–130 [CrossRef] [PubMed].
    [Google Scholar]
  12. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  13. Komagata K. , Suzuki K. . ( 1987; ). Lipids and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  14. Kovács N. . ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kwon K. K. , Woo J.-H. , Yang S.-H. , Kang J.-H. , Kang S. G. , Kim S.-J. , Sato T. , Kato C. . ( 2007; ). Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov.. Int J Syst Evol Microbiol 57:, 2207–2211. [CrossRef] [PubMed]
    [Google Scholar]
  16. Lai Q. , Yuan J. , Shao Z. . ( 2009; ). Altererythrobacter marinus sp. nov., isolated from deep seawater. . Int J Syst Evol Microbiol 59:, 2973–2976. [CrossRef] [PubMed]
    [Google Scholar]
  17. Lee K.-B. , Liu C.-T. , Anzai Y. , Kim H. , Aono T. , Oyaizu H. . ( 2005; ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55:, 1907–1919. [CrossRef] [PubMed]
    [Google Scholar]
  18. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  19. MIDI (1999). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc.
  20. Minnikin D. E. , Alshamaony L. , Goodfellow M. . ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. . J Gen Microbiol 88:, 200–204.[CrossRef]
    [Google Scholar]
  21. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  22. Ramesh Kumar N. , Nair S. , Langer S. , Busse H.-J. , Kämpfer P. . ( 2008; ). Altererythrobacter indicus sp. nov., isolated from wild rice (Porteresia coarctata Tateoka). . Int J Syst Evol Microbiol 58:, 839–844. [CrossRef] [PubMed]
    [Google Scholar]
  23. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  24. Seo S. H. , Lee S. D. . ( 2010; ). Altererythrobacter marensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 60:, 307–311. [CrossRef] [PubMed]
    [Google Scholar]
  25. Skerman V. B. D. . ( 1967; ). A Guide to the Identification of the Genera of Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  26. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Swofford, D. L. (1998). Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates.
  28. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  29. Yamaguchi S. , Yokoe M. . ( 2000; ). A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. . Appl Environ Microbiol 66:, 3337–3343. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yang S.-H. , Kwon K. K. , Lee H.-S. , Kim S.-J. . ( 2006; ). Shewanella spongiae sp. nov., isolated from a marine sponge. . Int J Syst Evol Microbiol 56:, 2879–2882 [CrossRef] [PubMed].
    [Google Scholar]
  31. Yoon J.-H. , Kang K. H. , Yeo S.-H. , Oh T.-K. . ( 2005; ). Erythrobacter luteolus sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 55:, 1167–1170 [CrossRef] [PubMed].
    [Google Scholar]
  32. Yoon J.-H. , Kang S.-J. , Lee M.-H. , Oh H. W. , Oh T.-K. . ( 2006; ). Porphyrobacter dokdonensis sp. nov., isolated from sea water. . Int J Syst Evol Microbiol 56:, 1079–1083 [CrossRef] [PubMed].
    [Google Scholar]
  33. Yurkov V. , Stackebrandt E. , Holmes A. , Fuerst J. A. , Hugenholtz P. , Golecki J. , Gad’on N. , Gorlenko V. M. , Kompantseva E. I. , Drews G. . ( 1994; ). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44:, 427–434 [CrossRef] [PubMed].
    [Google Scholar]
  34. ZoBell C. E. . ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. . J Mar Res 4:, 42–75.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.021196-0
Loading
/content/journal/ijsem/10.1099/ijs.0.021196-0
Loading

Data & Media loading...

Supplements

Polar lipid profiles of strains KYW48 (A, B) and KYW147 (C, D) after two-dimensional TLC detection with Zinzade reagent (A, C) and α-naphthol (B, D). DPG, Diphosphatidylglycerol; SGL, sphingoglycolipid; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PL1–3, unknown phospholipids; GL1–2, unknown glycolipids.

IMAGE

Field-emission scanning electron micrographs of cells of strains KYW48 (A) and KYW147 (B). Cells were grown on MB (Becton Dickinson) at 30 °C for 2 days. Bars, 1 µm.

IMAGE

Biolog GN2 MicroPlate results for strains KYW48 and KYW147 . [ PDF] (45 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error