sp. nov., isolated from desert sand Free

Abstract

A Gram-negative, aerobic, motile, like rod, strain 10-1-84, was isolated from a sand sample collected from the desert of Xinjiang, China. The isolate contained Q-10 as the predominant ubiquinone and Cω7, C, Cω7 and C 2-OH as the major fatty acids. The polyamine pattern contained predominantly -homospermidine. The main polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine and an unknown polar lipid. The DNA GC content was 63.3 mol%. 16S rRNA gene sequence similarity between strain 10-1-84 and the type strains of species of the genus ranged from 91.11 to 96.54 %. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain 10-1-84 belonged to the genus . On the basis of phylogenetic analysis and physiological and biochemical characterization, strain 10-1-84 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 10-1-84 ( = CCTCC AB 208035  = NRRL B-51332).

Funding
This study was supported by the:
  • R & D Infrastructure and Facility Development Program from the Ministry of Science and Technology of the People’s Republic of China (Award 2005DKA21208)
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2011-08-01
2024-03-29
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References

  1. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [CrossRef]
    [Google Scholar]
  2. Busse H.-J., Kämpfer P., Denner E. B. M. 1999; Chemotaxonomic characterisation of Sphingomonas . J Ind Microbiol Biotechnol 23:242–251 [View Article][PubMed]
    [Google Scholar]
  3. Busse H.-J., Denner E. B. M., Buczolits S., Salkinoja-Salonen M., Bennasar A., Kämpfer P. 2003; Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas . Int J Syst Evol Microbiol 53:1253–1260 [View Article][PubMed]
    [Google Scholar]
  4. Cappuccino J. G., Sherman N. 2002 Microbiology: a Laboratory Manual, 6th edn. Menlo Park, CA: Benjamin/Cummings;
    [Google Scholar]
  5. Cassidy M.-B., Lee H., Trevors J.-T., Zablotowicz R.-B. 1999; Chlorophenol and nitrophenol metabolism by Sphingomonas sp UG30. J Ind Microbiol Biotechnol 23:232–241 [View Article][PubMed]
    [Google Scholar]
  6. Chanal A., Chapon V., Benzerara K., Barakat M., Christen R., Achouak W., Barras F., Heulin T. 2006; The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria. Environ Microbiol 8:514–525 [View Article][PubMed]
    [Google Scholar]
  7. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  8. Huang H.-D., Wang W., Ma T., Li G.-Q., Liang F.-L., Liu R.-L. 2009; Sphingomonas sanxanigenens sp. nov., isolated from soil. Int J Syst Evol Microbiol 59:719–723 [View Article][PubMed]
    [Google Scholar]
  9. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  10. Lin Y.-C., Uemori K., de Briel D. A., Arunpairojana V., Yokota A. 2004; Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae . Int J Syst Evol Microbiol 54:1669–1676 [View Article][PubMed]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  12. Nigam A., Jit S., Lal R. 2010; Sphingomonas histidinilytica sp. nov., isolated from a hexachlorocyclohexane dump site. Int J Syst Evol Microbiol 60:1038–1043 [CrossRef]
    [Google Scholar]
  13. Roh S. W., Kim K.-H., Nam Y.-D., Chang H.-W., Kim M.-S., Oh H.-M., Bae J.-W. 2009; Sphingomonas aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 59:1359–1363 [View Article][PubMed]
    [Google Scholar]
  14. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. 2009; Sphingomonas japonica sp. nov., isolated from the marine crustacean Paralithodes camtschatica . Int J Syst Evol Microbiol 59:1179–1182 [View Article][PubMed]
    [Google Scholar]
  15. Scherer P., Kneifel H. 1983; Distribution of polyamines in methanogenic bacteria. J Bacteriol 154:1315–1322[PubMed]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Takeuchi M., Hiraishi A. 2001; Taxonomic significance of 2-hydroxy fatty acid profiles of the species in the genus Sphingomonas and related taxa. IFO Res Commun 20:72–82
    [Google Scholar]
  18. Takeuchi M., Kawai F., Shimada Y., Yokota A. 1993; Taxonomic study of polyethylene glycerol-utilizing bacteria: emended description of the genus Sphingomonas and new descriptions of Sphingomonas macrogoltabidus sp. nov., Sphingomonas sanguis sp. nov. and Sphingomonas terrae sp. nov.. Syst Appl Microbiol 16:227–238 [CrossRef]
    [Google Scholar]
  19. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417[PubMed]
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  22. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  23. White D. C., Sutton S. D., Ringelberg D. B. 1996; The genus Sphingomonas: physiology and ecology. Curr Opin Biotechnol 7:301–306 [View Article][PubMed]
    [Google Scholar]
  24. Wilson K. 1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 2.4.1–2.4.5 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Greene Publishing and Wiley-Interscience;
    [Google Scholar]
  25. Xie C. H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]
    [Google Scholar]
  26. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. 1990; Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34:99–119[PubMed] [CrossRef]
    [Google Scholar]
  27. Yabuuchi E., Kosako Y., Fujiwara N., Naka T., Matsunaga I., Ogura H., Kobayashi K. 2002; Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 52:1485–1496 [View Article][PubMed]
    [Google Scholar]
  28. Yoon J.-H., Lee M.-H., Kang S.-J., Lee S.-Y., Oh T.-K. 2006; Sphingomonas dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:2165–2169 [View Article][PubMed]
    [Google Scholar]
  29. Yoon J.-H., Park S., Kang S.-J., Kim W., Oh T.-K. 2009; Sphingomonas hankookensis sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 59:2788–2793 [View Article][PubMed]
    [Google Scholar]
  30. Zhang D.-C., Busse H.-J., Liu H.-C., Zhou Y.-G., Schinner F., Margesin R. 2011; Sphingomonas glacialis sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 61:587–591 [View Article][PubMed]
    [Google Scholar]
  31. Zipper C., Nickel K., Angst W., Kohler H.-P. 1996; Complete microbial degradation of both enantiomers of the chiral herbicide mecoprop [(RS)-2-(4-chloro-2-methylphenoxy)propionic acid] in an enantioselective manner by Sphingomonas herbicidovorans sp. nov.. Appl Environ Microbiol 62:4318–4322[PubMed]
    [Google Scholar]
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