1887

Abstract

A Gram-negative, aerobic bacterium, designated R-40509, was isolated from mucus of the reef builder coral () located in the São Sebastião Channel, São Paulo, Brazil. The strain was oxidase-positive and catalase-negative, and required Na for growth. Its phylogenetic position was in the genus and the closest related species were , and ; the isolate exhibited 16S rRNA gene sequence similarities of 97.5–98.0 % with the type strains of these species. 16S rRNA gene sequence similarities with other type strains of the genus were below 96 %. DNA–DNA hybridizations between strain R-40509 and the type strains of the phylogenetically closest species of the genus revealed less than 70 % DNA–DNA relatedness, supporting the novel species status of the strain. Phenotypic characterization revealed that the strain was able to grow at 15–42 °C and in medium containing up to 9 % NaCl. The isolate could be differentiated from phenotypically related species by several features, including its ability to utilize -alanine, -alanine, bromosuccinic acid, -hydroxybutyric acid and -ketovaleric acid, but not acetate or -arabinose. It produced acetoin (Voges–Proskauer), but did not have esterase lipase (C8) or catalase activities. It possessed C 7 (35 %), summed feature 3 (iso-C 2-OH and/or C 7; 25 %) and C (22 %) as major cellular fatty acids. The DNA G+C content was 58.5 mol%. The name sp. nov. is proposed to accommodate this novel isolate; the type strain is R-40509 (=LMG 25435 =CAIM 1449).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.021105-0
2011-01-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/1/60.html?itemId=/content/journal/ijsem/10.1099/ijs.0.021105-0&mimeType=html&fmt=ahah

References

  1. Baumann, P., Bowditch, R. D., Baumann, L. & Beaman, B. ( 1983; ). Taxonomy of marine Pseudomonas species: P. stanieri sp. nov.; P. perfectomarina sp. nov., nom. rev.; P. nautica; and P. doudoroffii. Int J Syst Bacteriol 33, 857–865.[CrossRef]
    [Google Scholar]
  2. Bowditch, R. D., Baumann, L. & Baumann, P. ( 1984; ). Description of Oceanospirillum kriegii sp. nov. and O. jannaschii sp. nov. and assignment of two species of Alteromonas to this genus as O. commune comb. nov. and O. vagum comb. nov. Curr Microbiol 10, 221–230.[CrossRef]
    [Google Scholar]
  3. Brown, B. E. & Bythell, J. C. ( 2005; ). Perspectives on mucus secretion in reef corals. Mar Ecol Prog Ser 296, 291–309.[CrossRef]
    [Google Scholar]
  4. Chang, H.-W., Nam, Y.-D., Kwon, H.-Y., Park, J. R., Lee, J.-S., Yoon, J.-H., An, K.-G. & Bae, J.-W. ( 2007; ). Marinobacterium halophilum sp. nov., a marine bacterium isolated from the Yellow Sea. Int J Syst Evol Microbiol 57, 77–80.[CrossRef]
    [Google Scholar]
  5. Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T. J., Higgins, D. G. & Thompson, J. D. ( 2003; ). Multiple sequence alignment with the clustal series of programs. Nucleic Acids Res 31, 3497–3500.[CrossRef]
    [Google Scholar]
  6. Chimetto, L. A., Brocchi, M., Thompson, C. C., Martins, R. C. R., Ramos, H. B. & Thompson, F. L. ( 2008; ). Vibrios dominate as culturable nitrogen-fixing bacteria of Brazilian coral Mussismilia hispida. Syst Appl Microbiol 31, 312–319.[CrossRef]
    [Google Scholar]
  7. Chimetto, L. A., Brocchi, M., Gondo, M., Thompson, C. C., Gomez-Gil, B. & Thompson, F. L. ( 2009; ). Genomic diversity of vibrios associated with the Brazilian coral Mussismilia hispida and its sympatric zoanthids (Palythoa caribaeorum, Palythoa variabilis and Zoanthus solanderi). J Appl Microbiol 106, 1818–1826.[CrossRef]
    [Google Scholar]
  8. Ducklow, H. W. & Mitchell, R. ( 1979; ). Composition of mucus released by coral reef coelenterates. Limnol Oceanogr 24, 706–714.[CrossRef]
    [Google Scholar]
  9. Eck, R. V. & Dayhoff, M. O. ( 1966; ). Atlas of Protein Sequence and Structure. Silver Springs, MD. : National Biomedical Research Foundation.
    [Google Scholar]
  10. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  11. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  12. González, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B. ( 1997; ). Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47, 369–376.[CrossRef]
    [Google Scholar]
  13. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  14. Huo, Y.-Y., Xu, X.-W., Cao, Y., Wang, C.-S., Zhu, X.-F., Oren, A. & Wu, M. ( 2009; ). Marinobacterium nitratireducens sp. nov. and Marinobacterium sediminicola sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 59, 1173–1178.[CrossRef]
    [Google Scholar]
  15. Huys, G., Vancanneyt, M., Coopman, R., Janssen, P., Falsen, E., Altwegg, M. & Kersters, K. ( 1994; ). Cellular fatty acid composition as a chemotaxonomic marker for the differentiation of phenospecies and hybridization groups in the genus Aeromonas. Int J Syst Bacteriol 44, 651–658.[CrossRef]
    [Google Scholar]
  16. Kim, H., Choo, Y.-J., Song, J., Lee, J.-S., Lee, K. C. & Cho, J.-C. ( 2007; ). Marinobacterium litorale sp. nov. in the order Oceanospirillales. Int J Syst Evol Microbiol 57, 1659–1662.[CrossRef]
    [Google Scholar]
  17. Kim, Y.-G., Jin, Y.-A., Hwang, C. Y. & Cho, B. C. ( 2008; ). Marinobacterium rhizophilum sp. nov., isolated from the rhizosphere of the coastal tidal-flat plant Suaeda japonica. Int J Syst Evol Microbiol 58, 164–167.[CrossRef]
    [Google Scholar]
  18. Kim, H., Oh, H.-M., Yang, S.-J., Lee, J.-S., Hong, J.-S. & Cho, J.-C. ( 2009a; ). Marinobacterium marisflavi sp. nov., isolated from a costal seawater. Curr Microbiol 58, 511–515.[CrossRef]
    [Google Scholar]
  19. Kim, S.-J., Park, S.-J., Yoon, D.-N., Park, B.-J., Choi, B.-R., Lee, D.-H., Roh, Y. & Rhee, S.-K. ( 2009b; ). Marinobacterium maritimum sp. nov., a marine bacterium isolated from Arctic sediment. Int J Syst Evol Microbiol 59, 3030–3034.[CrossRef]
    [Google Scholar]
  20. Kim, J. M., Lee, S. H., Jung, J. Y. & Jeon, C. O. ( 2010; ). Marinobacterium lutimaris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 60, 1828–1831.[CrossRef]
    [Google Scholar]
  21. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  22. Kovacs, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703.
    [Google Scholar]
  23. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  24. Reshef, L., Koren, O., Loya, Y., Zilber-Rosenberg, I. & Rosenberg, E. ( 2006; ). The coral probiotic hypothesis. Environ Microbiol 8, 2068–2073.[CrossRef]
    [Google Scholar]
  25. Ritchie, K. B. ( 2006; ). Regulation of microbial populations by coral surface mucus and mucus-associated bacteria. Mar Ecol Prog Ser 322, 1–14.[CrossRef]
    [Google Scholar]
  26. Rosenberg, E., Koren, O., Reshef, L., Efrony, R. & Zilber-Rosenberg, I. ( 2007; ). The role of microorganisms in coral health, disease and evolution. Nat Rev Microbiol 5, 355–362.[CrossRef]
    [Google Scholar]
  27. Rzhetsky, A. & Nei, M. ( 1992; ). A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9, 945–967.
    [Google Scholar]
  28. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  29. Satomi, M., Kimura, B., Hamada, T., Harayama, S. & Fujii, T. ( 2002; ). Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov. Int J Syst Evol Microbiol 52, 739–747.[CrossRef]
    [Google Scholar]
  30. Shnit-Orland, M. & Kushmaro, A. ( 2009; ). Coral mucus-associated bacteria: a possible first line of defense. FEMS Microbiol Ecol 67, 371–380.[CrossRef]
    [Google Scholar]
  31. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  32. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  33. Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J. ( 2001; ). Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplifed fragment length polymorphism. Syst Appl Microbiol 24, 520–538.[CrossRef]
    [Google Scholar]
  34. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  35. Wild, C., Rasheed, M., Werner, U., Franke, U., Johnstone, R. & Huettel, M. ( 2004; ). Degradation and mineralization of coral mucus in reef environments. Mar Ecol Prog Ser 267, 159–171.[CrossRef]
    [Google Scholar]
  36. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.021105-0
Loading
/content/journal/ijsem/10.1099/ijs.0.021105-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error