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Volume 61, Issue 1

sp. nov., isolated from mucus of coral () Free

Abstract

A Gram-negative, aerobic bacterium, designated R-40509, was isolated from mucus of the reef builder coral () located in the São Sebastião Channel, São Paulo, Brazil. The strain was oxidase-positive and catalase-negative, and required Na for growth. Its phylogenetic position was in the genus and the closest related species were , and ; the isolate exhibited 16S rRNA gene sequence similarities of 97.5–98.0 % with the type strains of these species. 16S rRNA gene sequence similarities with other type strains of the genus were below 96 %. DNA–DNA hybridizations between strain R-40509 and the type strains of the phylogenetically closest species of the genus revealed less than 70 % DNA–DNA relatedness, supporting the novel species status of the strain. Phenotypic characterization revealed that the strain was able to grow at 15–42 °C and in medium containing up to 9 % NaCl. The isolate could be differentiated from phenotypically related species by several features, including its ability to utilize -alanine, -alanine, bromosuccinic acid, -hydroxybutyric acid and -ketovaleric acid, but not acetate or -arabinose. It produced acetoin (Voges–Proskauer), but did not have esterase lipase (C8) or catalase activities. It possessed C 7 (35 %), summed feature 3 (iso-C 2-OH and/or C 7; 25 %) and C (22 %) as major cellular fatty acids. The DNA G+C content was 58.5 mol%. The name sp. nov. is proposed to accommodate this novel isolate; the type strain is R-40509 (=LMG 25435 =CAIM 1449).

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2011-01-01
2024-04-19
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Marinobacterium coralli sp. nov., isolated from mucus of coral (Mussismilia hispida)
Int J Syst Evol Microbiol 61, 60 (2011); https://doi.org/10.1099/ijs.0.021105-0
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