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Abstract

Two strains of Gram-variable, rod-shaped, endospore-forming, peritrichously flagellated, rod-shaped bacteria were isolated from a soil sample collected in Taejon City, Korea. The two strains (AM49 and AM141) were found to be members of the genus , on the basis of the results of phenotypic and phylogenetic analyses. Strains AM49 and AM141 were found to have a cell-wall peptidoglycan based on -diaminopimelic acid, MK-7 as their predominant menaquinone and anteiso-C as their major fatty acid. The DNA G+C contents of strains AM49 and AM141 were 48 and 47 mol%, respectively. The two strains formed distinct phylogenetic lineages within the radiation of the cluster comprising spp. and a coherent cluster with , , and . The level of 16S rDNA similarity between strains AM49 and AM141 was 97·6 %, and 16S rDNA similarity values between strains AM49 and AM141 and the type strains of other spp. ranged from 90·3 to 98·7 %. Levels of DNA–DNA similarity between the two strains and members of the genus indicated that strains AM49 and AM141 were distinguishable from each other and from four phylogenetically related spp. Therefore, on the basis of their phenotypic properties, phylogeny and genomic distinctiveness, it is proposed that strains AM49 and AM141 be placed in the genus as two distinct novel species, (AM49 =KCTC 0766BP =JCM 11465) and (AM141 =KCCM 41557 =JCM 11466).

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2003-01-01
2019-10-14
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The phylogenetic tree from which Fig. 2 was taken is available as PDF file when you click on the link below.



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