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Abstract

A moderately halophilic Gram-staining-negative bacterium, designated strain Y26, was isolated from a tidal flat of Taean coast in South Korea. Cells were strictly aerobic, motile cocci with a single flagellum and showed catalase- and oxidase-positive reactions. Growth of strain Y26 was observed at 15–35 °C (optimum 25–30 °C), pH 6.0–8.0 (optimum pH 6.5–7.5) and with 1.5–6.0 % (w/v) NaCl (optimum 2.0–3.0 %). The predominant fatty acids were C 7 (66.2 %), C (12.4 %) and C 3-OH (5.0 %) and the G+C content of the genomic DNA was 61.0 mol%. Strain Y26 contained ubiquinone-10 (Q-10) as the major respiratory quinone. Comparative 16S rRNA gene sequence analysis showed that strain Y26 formed a distinct phyletic lineage from other genera within the clade of the class and was most closely related to members of the genera , and with 93.8–94.6 % sequence similarity. On the basis of chemotaxonomic data and molecular properties, strain Y26 represents a novel genus, , within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is Y26 (=KACC 13705 =DSM 22009).

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2010-12-01
2021-10-22
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References

  1. Cho J. C., Giovannoni S. J. 2006; Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 56:855–859 [CrossRef]
    [Google Scholar]
  2. Choi D. H., Cho J.-C., Lanoil B. D., Giovannoni S. J., Cho B. C. 2007; Maribius salinus gen. nov., sp. nov., isolated from a solar saltern and Maribius pelagius sp. nov., cultured from the Sargasso Sea, belonging to the Roseobacter clade. Int J Syst Evol Microbiol 57:270–275 [CrossRef]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 2002 phylip (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  5. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L. 2004; Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54:93–97 [CrossRef]
    [Google Scholar]
  6. Kahng H.-Y., Chung B. S., Lee D.-H., Jung J. S., Park J. H., Jeon C. O. 2009; Cellulophaga tyrosinoxydans sp. nov., a tyrosinase-producing bacterium isolated from seawater. Int J Syst Evol Microbiol 59:654–657 [CrossRef]
    [Google Scholar]
  7. Kim Y.-G., Hwang C. Y., Cho B. C. 2008; Pelagicola litoralis gen. nov., sp. nov., isolated from coastal water in Korea. Int J Syst Evol Microbiol 58:2102–2106 [CrossRef]
    [Google Scholar]
  8. Kim J. M., Le N. T., Chung B. S., Park J. H., Bae J.-W., Madsen E. L., Jeon C. O. 2008; Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o -, m -, and p -xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 74:7313–7320 [CrossRef]
    [Google Scholar]
  9. Kim J. M., Lee S. H., Jung J. Y., Jeon C. O. 2010; Marinobacterium lutimaris sp. nov., isolated from a tidal-flat of the Yellow Sea. Int J Syst Evol Microbiol 60:1828–1831 [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  11. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Lee K., Choo Y.-J., Giovannoni S. J., Cho J.-C. 2007; Maritimibacter alkaliphilus gen. nov., sp. nov., a genome-sequenced marine bacterium of the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 57:1653–1658 [CrossRef]
    [Google Scholar]
  13. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O. 2006; Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. J Microbiol 44:155–161
    [Google Scholar]
  14. Martínez-Checa F., Quesada E., Martínez-Cánovas M. J., Llamas I., Béjar V. 2005; Palleronia marisminoris gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium belonging to the ‘ Alphaproteobacteria ’, isolated from a saline soil.. Int J Syst Evol Microbiol 55:2525–2530 [CrossRef]
    [Google Scholar]
  15. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
    [Google Scholar]
  16. Shiba T. 1991; Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a. Syst Appl Microbiol 14:140–145 [CrossRef]
    [Google Scholar]
  17. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. . In Methods for General and Molecular Bacteriology pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  19. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  20. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Mollica A., Faimali M., Vandamme P. 2009; Nautella italica gen. nov., sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 59:811–817 [CrossRef]
    [Google Scholar]
  21. Wang Y.-X., Wang Z.-G., Liu J.-H., Chen Y.-G., Zhang X.-X., Wen M.-L., Xu L.-H., Peng Q., Cui X.-L. 2009; Sediminimonas qiaohouensis gen. nov., sp. nov., a member of the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 59:1561–1567 [CrossRef]
    [Google Scholar]
  22. Yoon J.-H., Kang S.-J., Lee J.-S., Oh T.-K. 2009; Lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 59:48–52 [CrossRef]
    [Google Scholar]
  23. Zheng Q., Chen C., Yan X.-J., Wang Y.-N., Zeng Y.-H., Hao L.-K., He W.-H., Jiao N.-Z. 2010; Mameliella alba gen. nov., sp. nov. a marine bacterium of the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 60:953–957 [CrossRef]
    [Google Scholar]
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