1887

Abstract

Two novel cold-tolerant, Gram-stain-positive, motile, facultatively anaerobic bacterial strains, LI2 and LI3, were isolated from moss-covered soil from Livingston Island, Antarctica, near the Bulgarian station St Kliment Ohridski. A rod–coccus cycle was observed for both strains. 16S rRNA gene sequence analysis revealed an affiliation to the genus , with the highest similarity to and for strain LI2 (97.8 and 97.7 % similarity to the respective type strains) and to and for strain LI3 (97.4 and 97.3 % similarity to the respective type strains). The growth temperature range was −6 to 28 °C for LI2 and −6 to 24 °C for LI3, with an optimum at 16 °C for both strains. Growth occurred at 0–10 % (w/v) NaCl, with optimum growth at 0–1 % (w/v) for LI2 and 0.5–3 % (w/v) for LI3. The pH range for growth was pH 4–9.5 with an optimum of pH 8 for LI2 and pH 6.5 for LI3. The predominant fatty acids were anteiso-C, C and anteiso-C for LI2 and anteiso-C and C for LI3. Physiological and biochemical tests clearly differentiated strain LI2 from and and strain LI3 from and . Therefore, two novel species within the genus are proposed: sp. nov. (type strain LI2  = DSM 22825  = NCCB 100314) and sp. nov. (type strain LI3  = DSM 22826  = NCCB 100315).

Funding
This study was supported by the:
  • , Deutsche Forschungsgemeinschaft (DFG) , (Award WA 1554/4)
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2011-04-01
2020-10-27
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