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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 3

sp. nov., a chlorothalonil-degrading bacterium isolated from a long-term chlorothalonil-contaminated soil Free

Abstract

An aerobic, Gram-negative bacterial strain, designated CTN-1, capable of degrading chlorothalonil was isolated from a long-term chlorothalonil-contaminated soil in China, and was subjected to a polyphasic taxonomic investigation. Strain CTN-1 grew at 15–37 °C (optimum 28–30 °C) and at pH 6.0–9.0 (optimum pH 7.0–7.5). The G+C content of the total DNA was 67.1 mol%. Based on 16S rRNA gene sequence analysis, strain CTN-1 was related most closely to DSM 17634 (97.1 % similarity), DCY21 (95.7 %), Ko07 (95.5 %), LMG 8763 (95.3 %) and DSM 18481 (95.2 %). The novel strain showed less than 95.0 % 16S rRNA gene sequence similarity to the type strains of other species. The major cellular fatty acids of strain CNT-1 were iso-C (23.0 %), iso-C (21.4 %) and iso-C 9 (15.3 %). The major isoprenoid quinone was Q-8 (99 %), and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. These chemotaxonomic data supported the affiliation of strain CTN-1 to the genus Levels of DNA–DNA relatedness between strain CTN-1 and DSM 17634 were 34.6–36.1 %. Phylogenetic analysis based on 16S rRNA gene sequences, DNA–DNA hybridization data and biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain CTN-1 from recognized species of the genus . Strain CTN-1 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CTN-1 (=DSM 22393 =CGMCC 1.10136).

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2011-03-01
2024-04-18
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Lysobacter ruishenii sp. nov., a chlorothalonil-degrading bacterium isolated from a long-term chlorothalonil-contaminated soil
Int J Syst Evol Microbiol 61, 674 (2011); https://doi.org/10.1099/ijs.0.020990-0
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