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Abstract

Strain N75 was isolated from the surface of a copper-alloy 50 Euro cent coin collected from general circulation. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain N75 formed a distinct branch within the genus and placed it in the . Strain N75 showed 16S rRNA gene sequence similarities of 92.4–97.1 % to type strains of species of the genus . Strain N75 was a Gram-negative, non-spore-forming, non-motile coccoid, with an optimum growth temperature of about 30 °C; the strain did not grow at 5 or 37 °C. Strain N75 did not grow in medium containing NaCl. The major respiratory quinone was ubiquinone 10 (Q-10). The major fatty acids were unsaturated C 7/C 6 and C 7 (around 70 % of the total fatty acids); the third most abundant fatty acid was the hydroxylated C 2-OH. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine and an unknown aminolipid. The DNA G+C content was 72.8 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, we conclude that strain N75 represents a novel species of the genus , for which we propose the name sp. nov. (type strain N75 =LMG 25481 =CIP 110074).

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2011-03-01
2019-10-20
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