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Abstract

A novel hydrogenotrophic methanogen, designated strain MRE50, was isolated from a methanogenic consortium, which was originally established from an Italian rice field soil. Cells were non-motile rods, 1.3–2.8 μm long and 0.4–0.7 μm wide. Coccoid cells were also observed in cultures at the late-exponential phase of growth. Strain MRE50 grew at 37–55 °C (optimally at 45 °C), at pH 6–7.8 (optimally at pH 7.0) and in the presence of 0–20 g NaCl l. The isolate utilized H/CO and formate for growth and methane production. Phylogenetic analyses of the 16S rRNA gene and the methanogen-specific marker gene showed that strain MRE50 is affiliated with the order , previously known as uncultured archaeal group Rice Cluster I. Based on both 16S rRNA gene and gene sequences, strain MRE50 was related most closely to SANAE. Levels of sequence similarity were 92.5 and 86.1 %, respectively, indicating that strains MRE50 and SANAE represent different species within the genus . In addition, although these strains shared phenotypic properties including cell morphology and substrate utilization, they differed with respect to susceptibility to antibiotics, and temperature and NaCl ranges for growth. Given the phenotypic differences and the distinct phylogenetic placement of the new isolate relative to the type species of the genus , strain MRE50 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MRE50 (=NBRC 105507 =DSM 22066).

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2010-12-01
2019-10-21
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vol. , part 12, pp. 2918 - 2923

Photomicrographs of cells of SANAE . (a) Electron micrograph of a freeze-etched preparation showing a whole cell with a hexagonally ordered S-layer lattice; (b) transmission electron micrograph of a negatively stained cell. Bars, 500 nm.



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