A bacterial strain, designated V2M29T, was isolated from forest soil collected in the Changbai Mountains, Heilongjiang Province, China. Cells of strain V2M29T were Gram-positive, rod-shaped (0.5–0.8 μm in diameter and 1.5–2.0 μm in length), strictly aerobic and non-motile. Colonies produced on R2A agar plates were creamy, smooth, circular and 0.3–0.8 mm in diameter. Strain V2M29T grew at 14–35 °C (optimum, 29 °C), at pH 4.1–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–7.0 % (w/v) NaCl (optimum, 0–2.0 %). Strain V2M29T contained MK-8(H4) as the major respiratory quinone and iso-C15 : 0 (20.9 %), iso-C16 : 0 (18.5 %), C17 : 1ω8c (16.7 %) and 10-methyl-C17 : 0 (9.2 %) as the major cellular fatty acids. The cell-wall peptidoglycan type was based on meso-2,6-diaminopimelic acid and the acyl type of the muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylinositol and six unknown glycolipids were the major polar lipids. The G+C content of the DNA was 72.0 mol% (Tm). 16S rRNA gene sequence analysis indicated that strain V2M29T was phylogenetically related to members of the genus Phycicoccus, with sequence similarities ranging from 96.6–97.8 %. The DNA–DNA relatedness values of strain V2M29T to Phycicoccus dokdonensis DS-8T and Phycicoccus bigeumensis DSM 19264T were 32.5±3.3 % and 29.2±2.3 %, respectively. Based on these results, it is concluded that strain V2M29T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus cremeus sp. nov. is proposed, with V2M29T (=CGMCC 1.6963T =NBRC 104261T) as the type strain.
CollinsM. D.1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
DastagerS. G.,
LeeJ.-C.,
JuY.-J.,
ParkD.-J.,
KimC.-J.2008; Phycicoccus bigeumensis sp. nov., a mesophilic actinobacterium isolated from Bigeum Island, Korea. Int J Syst Evol Microbiol 58:2425–2428[CrossRef]
DongX.-Z.,
CaiM.-Y.
(editors) 2001; Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria pp 370–398 Beijing: : Science Press (in Chinese)
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
(editors) 1994Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
HußV. A. R.,
FestlH.,
SchleiferK. H.1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192[CrossRef]
KamekuraM.1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria pp 135–161 Edited by
VreelandR. H.,
HochsteinL. I.
Boca Raton, FL: CRC Press;
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118[CrossRef]
RossH. N. M.,
GrantW. D.,
HarrisJ. E.1985; Lipids in archaebacterial taxonomy. In Chemical Methods in Bacterial Systematics pp 289–300 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
UchidaK.,
AidaK.1984; An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134[CrossRef]
XinH.,
ItohT.,
ZhouP.,
SuzukiK.,
NakaseT.2001; Natronobacterium nitratireducens sp. nov., a haloalkaliphilic archaeon isolated from a soda lake in China. Int J Syst Evol Microbiol 51:1825–1829[CrossRef]
ZhangD.,
YangH.,
ZhangW.,
HuangZ.,
LiuS.-J.2003; Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53:1111–1114[CrossRef]