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Abstract

A bacterial strain, designated V2M29, was isolated from forest soil collected in the Changbai Mountains, Heilongjiang Province, China. Cells of strain V2M29 were Gram-positive, rod-shaped (0.5–0.8 μm in diameter and 1.5–2.0 μm in length), strictly aerobic and non-motile. Colonies produced on R2A agar plates were creamy, smooth, circular and 0.3–0.8 mm in diameter. Strain V2M29 grew at 14–35 °C (optimum, 29 °C), at pH 4.1–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–7.0 % (w/v) NaCl (optimum, 0–2.0 %). Strain V2M29 contained MK-8(H) as the major respiratory quinone and iso-C (20.9 %), iso-C (18.5 %), C 8 (16.7 %) and 10-methyl-C (9.2 %) as the major cellular fatty acids. The cell-wall peptidoglycan type was based on -2,6-diaminopimelic acid and the acyl type of the muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylinositol and six unknown glycolipids were the major polar lipids. The G+C content of the DNA was 72.0 mol% ( ). 16S rRNA gene sequence analysis indicated that strain V2M29 was phylogenetically related to members of the genus , with sequence similarities ranging from 96.6–97.8 %. The DNA–DNA relatedness values of strain V2M29 to DS-8 and DSM 19264 were 32.5±3.3 % and 29.2±2.3 %, respectively. Based on these results, it is concluded that strain V2M29 represents a novel species of the genus , for which the name sp. nov. is proposed, with V2M29 (=CGMCC 1.6963 =NBRC 104261) as the type strain.

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2011-01-01
2020-01-19
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Scanning electron micrograph of cells of strain V2M29 grown on R2A agar at 30 °C for 2 days. Bar, 10 µm.

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Polar lipids of strain V2M29 after separation by two-dimensional TLC. (a) Phospholipids, (b) glycolipids. DPG, diphosphatidylglycerol; PI, phosphatidylinositol; GL1–GL6, unknown glycolipids.

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Cellular fatty acid composition (%) of strain V2M29 and the type strains of other species of the genus . [ PDF] (42 KB)

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