A Gram-negative-staining, short-rod-shaped, floc-forming bacterium, designated strain RB3-7T, was isolated from a laboratory-scale activated sludge system treating coke plant effluent. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate was distantly related (≤95.8 % similarity) to Ottowia thiooxydans K11T within the family Comamonadaceae. Strain RB3-7T was catalase- and oxidase-positive and non-motile. The predominant fatty acids were C16 : 0, cyclo C17 : 0, C18 : 1ω7c and C16 : 1ω7c, and the major respiratory quinone was Q-8. The G+C content of the genomic DNA of strain RB3-7T was 68.5 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain RB3-7T is considered to represent a novel species of the genus Ottowia, for which the name Ottowia pentelensis sp. nov. is proposed. The type strain is RB3-7T ( = DSM 21699T = NCAIM B 02336T).
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Funding
This study was supported by the:
Hungarian Economic Competitiveness Operational Program
(Award GVOP-3.2.2-2004-07-0019/3.0)
Ministry of Education and Culture, Hungary
(Award DFÖ 0051/2009)
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
ChunJ.,
LeeJ. H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY. W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed][CrossRef]
GrothI.,
SchumannP.,
RaineyF. A.,
MartinK.,
SchuetzeB.,
AugstenK.1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [View Article][PubMed]
KatayamaY.,
NaraharaY.,
InoueY.,
AmanoF.,
KanagawaT.,
KuraishiH.1992; A thiocyanate hydrolase of Thiobacillus thioparus. A novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate. J Biol Chem 267:9170–9175[PubMed]
KämpferP.,
SteiofM.,
DottW.1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [View Article]
KämpferP.,
KroppenstedtR. M.1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
MalikK. A.,
SchlegelH. G.1981; Chemolithoautotrophic growth of bacteria able to grow under N2-fixing conditions. FEMS Microbiol Lett 11:63–67 [View Article]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
SiposR.,
SzékelyA. J.,
PalatinszkyM.,
RévészS.,
MárialigetiK.,
NikolauszM.2007; Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis. FEMS Microbiol Ecol 60:341–350 [View Article][PubMed]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
SpringS.,
JäckelU.,
WagnerM.,
KämpferP.2004; Ottowia thiooxydans gen. nov., sp. nov., a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen. nov., comb. nov. Int J Syst Evol Microbiol 54:99–106 [View Article][PubMed]
SteadD. E.,
SellwoodJ. E.,
WilsonJ.,
VineyI.1992; Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Microbiol 72:315–321 [View Article]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
TindallB. J.1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130[CrossRef]
WenA.,
FeganM.,
HaywardC.,
ChakrabortyS.,
SlyL. I.1999; Phylogenetic relationships among members of the Comamonadaceae, and description of Delftia acidovorans (den Dooren de Jong 1926 and Tamaoka et al. 1987) gen. nov., comb. nov.. Int J Syst Bacteriol 49:567–576 [View Article][PubMed]