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A Gram-negative-staining, short-rod-shaped, floc-forming bacterium, designated strain RB3-7T, was isolated from a laboratory-scale activated sludge system treating coke plant effluent. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate was distantly related (≤95.8 % similarity) to Ottowia thiooxydans K11T within the family Comamonadaceae. Strain RB3-7T was catalase- and oxidase-positive and non-motile. The predominant fatty acids were C16 : 0, cyclo C17 : 0, C18 : 1ω7c and C16 : 1ω7c, and the major respiratory quinone was Q-8. The G+C content of the genomic DNA of strain RB3-7T was 68.5 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain RB3-7T is considered to represent a novel species of the genus Ottowia, for which the name Ottowia pentelensis sp. nov. is proposed. The type strain is RB3-7T ( = DSM 21699T = NCAIM B 02336T).
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