1887

Abstract

A Gram-positive actinobacterium, designated M24DS4, was isolated from a soil sample collected from Aomi, Tokyo, Japan, using the membrane-filter method. Strain M24DS4 exhibited low 16S rRNA gene sequence similarity (96.1 %) with NBRC 100760. Cell hydrolysates contained the -isomer of diaminopimelic acid and the predominant quinones were MK-9(H) and MK-9(H). The genomic DNA G+C content was 75 mol%. Comparison of the characteristics of strain M24DS4 and related members of the genus with validly published names showed that the strain represents a novel species of the genus, for which the name sp. nov. is proposed. The type strain is M24DS4 ( = NBRC 106164  = KACC 14925).

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2011-04-01
2019-10-17
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Brosius J. , Palmer M. L. , Kennedy P. J. , Noller H. F. . ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75:, 4801–4805. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bull A. T. , Ward A. C. , Goodfellow M. . ( 2000; ). Search and discovery strategies for biotechnology: the paradigm shift. . Microbiol Mol Biol Rev 64:, 573–606. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Hayakawa M. , Nonomura H. . ( 1987; ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  7. Hirsch C. F. , Christensen D. L. . ( 1983; ). Novel method for selective isolation of actinomycetes. . Appl Environ Microbiol 46:, 925–929.[PubMed]
    [Google Scholar]
  8. McVeigh H. P. , Divers M. , Warwick S. , Munro J. , Embley T. M. . ( 1994; ). Exploration of actinomycete diversity using ribosomal RNA sequences. . Biotechnologia 7–8:, 253–260.
    [Google Scholar]
  9. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  10. Minamisawa K. . ( 1990; ). Division of rhizobitoxine-producing and hydrogen-uptake positive strains of Bradyrhizobium japonicum by nifDKE sequence divergence. . Plant Cell Physiol 31:, 81–89.
    [Google Scholar]
  11. Newman D. J. , Hill R. T. . ( 2006; ). New drugs from marine microbes: the tide is turning. . J Ind Microbiol Biotechnol 33:, 539–544. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ping X. , Takahashi Y. , Seino A. , Iwai Y. , Ōmura S. . ( 2004; ). Streptomyces scabrisporus sp. nov.. Int J Syst Evol Microbiol 54:, 577–581. [CrossRef] [PubMed]
    [Google Scholar]
  13. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  15. Stackebrandt E. , Ebers J. . ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33:, 152–155.
    [Google Scholar]
  16. Swofford D. L. . ( 2000; ). PAUP*: phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinaur Associates..
    [Google Scholar]
  17. Tamura T. , Nakagaito Y. , Nishii T. , Hasegawa T. , Stackebrandt E. , Yokota A. . ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:, 193–203. [CrossRef] [PubMed]
    [Google Scholar]
  18. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
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vol. , part 4, pp. 947 - 950

Scanning electron micrograph showing the spore chain morphology of sp. nov. M24DS4 after 10 days on ISP 2 medium at 28 °C. Bar, 5 µm.

Fatty acid content of sp. nov. M24DS4



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