1887

Abstract

A Gram-positive actinobacterium, designated M24DS4, was isolated from a soil sample collected from Aomi, Tokyo, Japan, using the membrane-filter method. Strain M24DS4 exhibited low 16S rRNA gene sequence similarity (96.1 %) with NBRC 100760. Cell hydrolysates contained the -isomer of diaminopimelic acid and the predominant quinones were MK-9(H) and MK-9(H). The genomic DNA G+C content was 75 mol%. Comparison of the characteristics of strain M24DS4 and related members of the genus with validly published names showed that the strain represents a novel species of the genus, for which the name sp. nov. is proposed. The type strain is M24DS4 ( = NBRC 106164  = KACC 14925).

Funding
This study was supported by the:
  • , New Energy and Industrial Technology Department Organization (NEDO) of Japan
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2011-04-01
2020-07-15
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef][PubMed]
    [Google Scholar]
  3. Bull A. T., Ward A. C., Goodfellow M. 2000; Search and discovery strategies for biotechnology: the paradigm shift. Microbiol Mol Biol Rev 64:573–606 [CrossRef][PubMed]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [CrossRef]
    [Google Scholar]
  7. Hirsch C. F., Christensen D. L. 1983; Novel method for selective isolation of actinomycetes. Appl Environ Microbiol 46:925–929[PubMed]
    [Google Scholar]
  8. McVeigh H. P., Divers M., Warwick S., Munro J., Embley T. M. 1994; Exploration of actinomycete diversity using ribosomal RNA sequences. Biotechnologia 7–8:253–260
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  10. Minamisawa K. 1990; Division of rhizobitoxine-producing and hydrogen-uptake positive strains of Bradyrhizobium japonicum by nifDKE sequence divergence. Plant Cell Physiol 31:81–89
    [Google Scholar]
  11. Newman D. J., Hill R. T. 2006; New drugs from marine microbes: the tide is turning. J Ind Microbiol Biotechnol 33:539–544 [CrossRef][PubMed]
    [Google Scholar]
  12. Ping X., Takahashi Y., Seino A., Iwai Y., Ōmura S. 2004; Streptomyces scabrisporus sp. nov.. Int J Syst Evol Microbiol 54:577–581 [CrossRef][PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  14. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  15. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  16. Swofford D. L. 2000; PAUP*: phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinaur Associates..
    [Google Scholar]
  17. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. 1994; A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:193–203 [CrossRef][PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
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