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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 61, Issue 4

gen. nov., sp. nov., a halophilic archaeon isolated from a seawater aquarium Free

Abstract

A novel halophilic archaeon, strain KeC-11, was isolated from a seawater aquarium at the Ocean Research Institute, University of Tokyo, Japan. The strain was aerobic, Gram-negative and chemo-organotrophic, growing optimally at 37 °C, at pH 7.0–8.0 and in 2.7 M (16 %) NaCl. The strain required at least 10 mM magnesium ions for growth. Cells of strain KeC-11 were non-motile and generally irregular coccoids or discoids. The DNA G+C content of the isolate was 67.7 mol%. Phylogenetic tree reconstructions indicated that it was distantly related to the other recognized members of the family , with the closest relative being Gabara (91 % sequence similarity). The strain contained CC and CC diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, a glycolipid chromatographically identical to the glycosyl-mannosyl-glucosyl diether (TGD-2) and at least one unidentified glycolipid. Phenotypic characterization and phylogenetic data support the placement of isolate KeC-11 in a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed; the type strain is KeC-11 ( = JCM 16495  = KCTC 4074).

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Funding
This study was supported by the:
  • Ministry of Education, Culture, Sports, Science and Technology of Japan (Award 18201003)
© 2011 IUMS
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2011-04-01
2025-05-16
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Halomarina oriensis gen. nov., sp. nov., a halophilic archaeon isolated from a seawater aquarium
Int J Syst Evol Microbiol 61, 942 (2011); https://doi.org/10.1099/ijs.0.020677-0
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