1887

Abstract

A novel actinobacterial strain, designated CPCC 201356, was isolated from a rhizosphere soil sample of the medicinal plant and subjected to a polyphasic taxonomic analysis. Good growth occurred at 20–32 °C, at pH 7.0–7.5 and with 0–1 % (w/v) NaCl. Colonies on R2A and ISP 2 agar were light red to red, round and lacked aerial mycelium; cells adhered to the agar. The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H) and MK-9. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and two unknown phospholipids. The major cellular fatty acids were iso-C, iso-C and C 8. The G+C content of the genomic DNA was 72.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CPCC 201356 belonged to the family and consistently formed a distinct sub-branch with DSM 43160. The organism showed 16S rRNA gene sequence similarity of 97.7 % with DSM 43160. DNA–DNA hybridization between strain CPCC 201356 and DSM 43160 was 17.4 %. On the basis of evidence from this polyphasic taxonomic study, a novel species, sp. nov., is proposed; the type strain is CPCC 201356 (=DSM 45317 =CCM 7619).

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2011-01-01
2021-03-07
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