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Volume 61, Issue 4

gen. nov., sp. nov., a thermophilic sulfate-reducing bacterium from a terrestrial hot spring Free

Abstract

A thermophilic, sulfate-reducing bacterium, designated strain USBA-053, was isolated from a terrestrial hot spring located at a height of 2500 m in the Colombian Andes (5° 45′ 33.29″ N 73° 6′ 49.89″ W), Colombia. Cells of strain USBA-053 were oval- to rod-shaped, Gram-negative and motile by means of a single polar flagellum. The strain grew autotrophically with H as the electron donor and heterotrophically on formate, propionate, butyrate, valerate, isovalerate, lactate, pyruvate, ethanol, glycerol, serine and hexadecanoic acid in the presence of sulfate as the terminal electron acceptor. The main end products from lactate degradation, in the presence of sulfate, were acetate, CO and HS. Strain USBA-053 fermented pyruvate in the absence of sulfate and grew optimally at 57 °C (growth temperature ranged from 50 °C to 62 °C) and pH 6.8 (growth pH ranged from 5.7 to 7.7). The novel strain was slightly halophilic and grew in NaCl concentrations ranging from 5 to 30 g l, with an optimum at 25 g l NaCl. Sulfate, thiosulfate and sulfite were used as electron acceptors, but not elemental sulfur, nitrate or nitrite. The G+C content of the genomic DNA was 56±1 mol%. 16S rRNA gene sequence analysis indicated that strain USBA-053 was a member of the class , with MT-96 as the closest relative (93 % gene sequence similarity). On the basis of physiological characteristics and phylogenetic analysis, it is suggested that strain USBA-053 represents a new genus and novel species for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is USBA-053 ( = KCTC 5670 = DSM 22027).

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Funding
This study was supported by the:
  • IFS (International Foundation for Science)
  • Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología (Colciencias)
© 2011 IUMS
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2011-04-01
2024-12-14
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Desulfosoma caldarium gen. nov., sp. nov., a thermophilic sulfate-reducing bacterium from a terrestrial hot spring
Int J Syst Evol Microbiol 61, 732 (2011); https://doi.org/10.1099/ijs.0.020586-0
/content/journal/ijsem/10.1099/ijs.0.020586-0
/content/journal/ijsem/10.1099/ijs.0.020586-0
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