In order to investigate the genetic diversity of rhizobia associated with various exotic and invasive species in tropical mainland China, 116 bacterial isolates were obtained from Mimosa root nodules collected from Sishuangbanna and Yuanjiang districts of Yunnan province. Isolated rhizobia were characterized by RFLP analysis of 16S rRNA genes, SDS-PAGE of whole-cell proteins and BOX-PCR. Most of the isolated strains were identified as β-rhizobia belonging to diverse populations of Burkholderia and Cupriavidus, and the phylogenetic relationships of their 16S rRNA gene sequences showed that they were closely related to one of four β-rhizobia species: Burkholderia phymatum, B. mimosarum, B. caribensis or Cupriavidus taiwanensis. Additionally, among the 116 isolates, 53 different whole-cell SDS-PAGE profiles and 30 distinct BOX-PCR genotypic patterns were detected, which demonstrated the genetic and phenotypic diversity found within these Burkholderia and Cupriavidus strains. To the best of our knowledge, this is the first report that β-rhizobia are extant and possibly widespread on the Chinese mainland and nodulate easily with Mimosa plants. We also find it especially interesting that this appears to be the first report from mainland China of Cupriavidus symbionts of Mimosa. These records enrich our knowledge and understanding of the geographical distribution and diversity of these bacteria.
AndamC. P.,
MondoS. J.,
ParkerM. A.2007; Monophyly of nodA and nifH genes across Texan and Costa Rican populations of Cupriavidus nodule symbionts. Appl Environ Microbiol 73:4686–4690[CrossRef]
BarrettC. F.,
ParkerM. A.2005; Prevalence of Burkholderia sp. nodule symbionts on four mimosoid legumes from Barro Colorado Island, Panama. Syst Appl Microbiol 28:57–65[CrossRef]
BarrettC. F.,
ParkerM. A.2006; Coexistence of Burkholderia , Cupriavidus , and Rhizobium sp. nodule bacteria on two Mimosa spp. in Costa Rica. Appl Environ Microbiol 72:1198–1206[CrossRef]
ChenW. M.,
LaevensS.,
LeeT. M.,
CoenyeT.,
De VosP.,
MergeayM.,
VandammeP.2001; Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51:1729–1735[CrossRef]
ChenW. M.,
de FariaS.,
StraliottoM. R.,
PitardR. M.,
Simões-AraùjoJ. L.,
ChouJ. H.,
ChouY. J.,
BarriosE.,
PrescottA. R. & other authors (2005a). Proof that Burkholderia strains form effective symbioses with legumes: a study of novel Mimosa nodulating strains from South America. Appl Environ Microbiol 71:7461–7471[CrossRef]
ChenW. M.,
JamesE. K.,
ChouJ. H.,
SheuS. Y.,
YangS. Z.,
SprentJ. I.2005b; β -Rhizobia from Mimosa pigra , a newly discovered invasive plant in Taiwan. New Phytol 168:661–675[CrossRef]
ChenW.-M.,
JamesE. K.,
CoenyeT.,
ChouJ.-H.,
BarriosE.,
de FariaS. M.,
ElliottG. N.,
SheuS.-Y.,
SprentJ. I.,
VandammeP.2006; Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America. Int J Syst Evol Microbiol 56:1847–1851[CrossRef]
ChenW.-M.,
de FariaS. M.,
JamesE. K.,
ElliottG. N.,
LinK.-Y.,
ChouJ.-H.,
SheuS.-Y.,
CnockaertM.,
SprentJ. I.,
VandammeP.2007; Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella
. Int J Syst Evol Microbiol 57:1055–1059[CrossRef]
ChenW.-M.,
de FariaS. M.,
ChouJ.-H.,
JamesE. K.,
ElliottG. N.,
SprentJ. I.,
BontempsC.,
YoungJ. P. W.,
VandammeP.2008; Burkholderia sabiae sp. nov., isolated from root nodules of Mimosa caesalpiniifolia
. Int J Syst Evol Microbiol 58:2174–2179[CrossRef]
ElliottG. N.,
ChenW. M.,
ChouJ. H.,
WangH. C.,
SheuS. Y.,
PerinL.,
ReisV. M.,
MoulinL.,
SimonM. F. & other authors (2007b). Burkholderia phymatum is a highly effective nitrogen-fixing symbiont of Mimosa spp. and fixes nitrogen ex planta
. New Phytol 173:168–180[CrossRef]
ElliottG. N.,
ChouJ. H.,
ChenW. M.,
BloembergG. V.,
BontempsC.,
Martínez-RomeroE.,
VelázquezE.,
YoungJ. P.,
SprentJ. I.,
JamesE. K.2009; Burkholderia spp. are the most competitive symbionts of Mimosa , particularly under N-limited conditions. Environ Microbiol 11:762–778[CrossRef]
GaoJ. L.,
TerefeworkZ.,
ChenW.,
LindströmK.2001; Genetic diversity of rhizobia isolated from Astragalus adsurgens growing in different geographical regions of China. J Biotechnol 91:155–168[CrossRef]
GarauG.,
YatesR. J.,
DeianaP.,
HowiesonJ. G.2009; Novel strains of nodulating Burkholderia have a role in nitrogen fixation with papilionoid herbaceous legumes adapted to acid, infertile soils. Soil Biol Biochem 41:125–134[CrossRef]
GuanZ.-B.,
DengW.-H.,
HuangZ.-L.,
HuangN.-Y.,
AiL.,
LiC.-Y.2006; A preliminary investigation on the alien invasive plants in Xishuangbanna. Trop Agric Sci Technol 29:435–38 (in Chinese)
JamesE. K.,
CrawfordR. M. M.1998; Effect of oxygen availability on nitrogen fixation by two Lotus species under flooded conditions. J Exp Bot 49:599–609[CrossRef]
LaguerreG.,
AllardM. R.,
RevoyF.,
AmargerN.1994; Rapid identification of rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes. Appl Environ Microbiol 60:56–63
LiuX. Y.,
WangE. T.,
LiY.,
ChenW. X.2007; Diverse bacteria isolated from root nodules of Trifolium , Crotalaria and Mimosa grown in the subtropical regions of China. Arch Microbiol 188:1–14[CrossRef]
ParkerM. A.,
WurtzA. K.,
PaynterQ.2007; Nodule symbiosis of invasive Mimosa pigra in Australia and in ancestral habitats: a comparative analysis. Biol Invasions 9:127–138[CrossRef]
TanZ. Y.,
XuX. D.,
WangE. T.,
GaoJ. L.,
Martínez-RomeroE.,
ChenW. X.1997; Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia. Int J Syst Bacteriol 47:874–879[CrossRef]
ThompsonJ. D.,
GibsonT.,
PlewniakJ. F.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]