1887

Abstract

An actinomycete strain (YIM 90004) was isolated from a saline soil sample from the Xinjiang Province, People's Republic of China. The strain displayed abundant aerial and substrate mycelia, and short spore chains were borne on the aerial mycelium. The spore chains were composed of non-motile, smooth-surfaced, rod-shaped spores. The cell wall of strain YIM 90004 contained mainly -diaminopimelic acid, alanine and glutamic acid (cell wall type III). Glucose, ribose, galactose, xylose and arabinose were the whole-cell sugars of the strain. The predominant phospholipids were phosphatidylinositol and phosphatidylglycerol; MK-10(H) and MK-10(H) were the predominant menaquinones. The DNA G+C content of strain YIM 90004 was 74·3 mol%. Chemotaxonomic properties and 16S rDNA analysis placed strain YIM 90004 in the genus . On the basis of phenotypic and phylogenetic analyses and DNA–DNA hybridization results, strain YIM 90004 (=CCRC 16285 =CCTCC AA99004 =DSM 44589) is considered to represent a novel species of the genus , for which the name is proposed.

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2003-01-01
2019-12-07
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References

  1. Al-Tai, A. M. & Ruan, J.-S. ( 1994; ). Nocardiopsis halophila sp. nov., a new halophilic actinomycete isolated from soil. Int J Syst Bacteriol 44, 474–478.[CrossRef]
    [Google Scholar]
  2. Al-Zarban, S. S., Abbas, I., Al-Musallam, A. A., Steiner, U., Stackebrandt, E. & Kroppenstedt, R. M. ( 2002; ). Nocardiopsis halotolerans sp. nov., isolated from salt marsh soil in Kuwait. Int J Syst Evol Microbiol 52, 525–529.
    [Google Scholar]
  3. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  4. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in bacterial classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. London: Academic Press.
  5. Cui, X. L., Mao, P. H, Tseng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L. ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  6. Evtushenko, L. I., Taran, V. V., Akimov, V. N., Kroppenstedt, R. M., Tiedje, J. M. & Stackebrandt, E. ( 2000; ). Nocardiopsis tropica sp. nov., Nocardiopsis trehalosi sp. nov., nom. rev. and Nocardiopsis dassonvillei subsp. albirubida subsp. nov., comb. nov. Int J Syst Evol Microbiol 50, 73–81.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P. & Ward, J. M. ( 1985; ). Preparation of chromosomal, plasmid and phage DNA. In Genetic Manipulation of Streptomyces – a Laboratory Manual, pp. 79–80. Norwich, UK: F. Crowe & Sons.
  9. Jiang, C. L. & Xu, L. H. ( 1995; ). Actinomycete Taxonomy, pp. 62–63, 119–184. Kunming, China: Yunnan University Press.
  10. Kelly, K. L. ( 1964; ). Inter-Society Color Council–National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  13. Kroppenstedt, R. M. ( 1992; ). The genus Nocardiopsis. In The Prokaryotes, pp. 1139–1156. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer-Verlag.
  14. Lechevalier, M. P. & Lechevalier, H. A. ( 1980; ). The Chemotaxonomy of Actinomycetes. Actinomycete Taxonomy, pp. 227–291. Edited by X. Dietz & Y. Thayer. Arlington, VA: Society for Industrial Microbiology.
  15. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  16. Peltola, J. S., Andersson, M. A., Kämpfer, P., Auling, G., Kroppenstedt, R. M., Busse, H.-J., Salkinoja-Salonen, M. S. & Rainey, F. A. ( 2001; ). Isolation of toxigenic Nocardiopsis strains from indoor environments and description of two new Nocardiopsis species, N. exhalans sp. nov. and N. umidischolae sp. nov. Appl Environ Microbiol 67, 4293–4304.[CrossRef]
    [Google Scholar]
  17. Saddler, G. S., Tavecchia, P., Lociure, S., Zand, M., Colombo, L. & Selva, E. ( 1991; ). Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14, 185–191.[CrossRef]
    [Google Scholar]
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  20. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  21. Takahashi, T., Egusa, H. & Deng, B. ( 1992; ). Quantitative analysis for madurose and other sugars in small amount of actinomycete whole cells by gas-liquid chromatography. Actinomycetologica 6, 69–78.[CrossRef]
    [Google Scholar]
  22. Williams, S. T., Goodfellow, M. & Alderson, G. ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  23. Yassin, A. F., Galinski, E. A., Wohlfarth, A., Jahnke, K.-D., Schaal, K. P. & Trüper, H. G. ( 1993; ). A new actinomycete species, Nocardiopsis lucentensis sp. nov. Int J Syst Bacteriol 43, 266–271.[CrossRef]
    [Google Scholar]
  24. Yassin, A. F., Rainey, F. A., Burghardt, J., Gierth, D., Ungerechts, J., Lux, l., Seifert, P., Bal, C. & Schaal, K. P. ( 1997; ). Description of Nocardiopsis synnemataformans sp. nov., elevation of Nocardiopsis alba subsp. prasina to Nocardiopsis prasina comb. nov., and designation of Nocardiopsis antarctica and Nocardiopsis alborubida as later subjective synonyms of Nocardiopsis dassonvillei. Int J Syst Bacteriol 47, 983–988.[CrossRef]
    [Google Scholar]
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