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Abstract

A Gram-stain-positive, non-endospore-forming actinobacterium (CC-12301) was isolated from soil attached to a spawn used in the laboratory to grow the edible mushroom . Based on 16S rRNA gene sequence similarities, strain CC-12301 was shown to belong to the genus and was most closely related to the type strains of (97.6 % similarity), (97.5 %), (97.5 %) and (97.4 %). The quinone system was determined to consist predominantly of menaquinone MK-9(H), minor amounts of MK-8(H) and MK-7(H). The polar lipid profile consisted of the major compounds diphosphatidylglycerol and phosphatidylethanolamine, moderate amounts of two phosphatidylinositol mannosides and phosphatidylinositol and minor amounts of phosphatidylglycerol, three unidentified glycolipids, two phosphoglycolipids and a phospholipid. Mycolic acids were present. These chemotaxonomic traits and the major fatty acids, which were C 9, C, C and tuberculostearic acid (10-methyl C), supported the affiliation of strain CC-12301 to the genus . The results of physiological and biochemical tests allowed clear phenotypic differentiation of strain CC-12301 from the most closely related species. Strain CC-12301 therefore represents a novel species, for which the name sp. nov. is proposed, with the type strain CC-12301 (=DSM 45298 =CCM 7727).

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2011-01-01
2019-12-13
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Polar lipid profiles of strain CC-12301 (a), DSM 44015 (b) and DSM 43249 (c) separated by two-dimensional TLC and detected with molybdatophosphoric acid. DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PIM(s), phosphatidylinositol mannoside(s); GL1–4, unidentified glycolipids; PGL1, 2, unidentified phosphoglycolipids ; PL1, 2, unidentified phospholipids; L1–12, unidentified lipids not stained with any of the specific spray reagents applied (molybdenum blue, ninhydrin and α-naphthol). The first dimension is horizontal.

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