1887

Abstract

A Gram-negative, non-motile, rod-shaped bacterial strain, DS-58, was isolated from a soil sample from Dokdo, an island of Korea, and its taxonomic position was investigated. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-58 fell within the family . The isolate showed 96.9 % 16S rRNA gene sequence similarity with its closest phylogenetic neighbour, GH41-7, and 93.4–95.7 % 16S rRNA gene sequence similarity with other members of the genus . Strain DS-58 contained Q-8 as the predominant ubiquinone and iso-C, iso-C and iso-Cω9 as the major fatty acids. The DNA G+C content was 68.1 mol%. Strain DS-58 could be distinguished phenotypically from type strains of closely related species of the genus and phylogenetically from all members of the genus . On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DS-58 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-58 ( = KCTC 12822  = DSM 17958).

Funding
This study was supported by the:
  • , 21C Frontier program of Microbial Genomics and Applications , (Award MG05-0401-2-0)
  • , Ministry of Education, Science and Technology (MEST)
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2011-05-01
2020-08-10
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References

  1. Aslam Z., Yasir M., Jeon C. O., Chung Y. R. 2009; Lysobacter oryzae sp. nov., isolated from the rhizosphere of rice (Oryza sativa L.). Int J Syst Evol Microbiol 59:675–680 [CrossRef][PubMed]
    [Google Scholar]
  2. Bae H.-S., Im W.-T., Lee S.-T. 2005; Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 55:1155–1161 [CrossRef][PubMed]
    [Google Scholar]
  3. Christensen P., Cook F. D. 1978; Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. Int J Syst Bacteriol 28:367–393 [CrossRef]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Euzéby J. P. 1997; List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 [CrossRef][PubMed]
    [Google Scholar]
  6. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  7. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
    [Google Scholar]
  8. Lee J. W., Im W.-T., Kim M. K., Yang D.-C. 2006; Lysobacter koreensis sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 56:231–235 [CrossRef][PubMed]
    [Google Scholar]
  9. Park J. H., Kim R., Aslam Z., Jeon C. O., Chung Y. R. 2008; Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter . Int J Syst Evol Microbiol 58:387–392 [CrossRef][PubMed]
    [Google Scholar]
  10. Romanenko L. A., Uchino M., Tanaka N., Frolova G. M., Mikhailov V. V. 2008; Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge. Int J Syst Evol Microbiol 58:370–374 [CrossRef][PubMed]
    [Google Scholar]
  11. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI, Inc.
  12. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  13. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  14. Ten L. N., Jung H.-M., Im W.-T., Yoo S.-A., Oh H.-M., Lee S.-T. 2009; Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 59:958–963 [CrossRef][PubMed]
    [Google Scholar]
  15. Wang Y., Dai J., Zhang L., Luo X., Li Y., Chen G., Tang Y., Meng Y., Fang C. 2009; Lysobacter ximonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 59:786–789 [CrossRef][PubMed]
    [Google Scholar]
  16. Weon H.-Y., Kim B.-Y., Baek Y.-K., Yoo S.-H., Kwon S.-W., Stackebrandt E., Go S.-J. 2006; Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. Int J Syst Evol Microbiol 56:947–951 [CrossRef][PubMed]
    [Google Scholar]
  17. Weon H.-Y., Kim B.-Y., Kim M.-K., Yoo S.-H., Kwon S.-W., Go S.-J., Stackebrandt E. 2007; Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. Int J Syst Evol Microbiol 57:548–551 [CrossRef][PubMed]
    [Google Scholar]
  18. Yassin A. F., Chen W.-M., Hupfer H., Siering C., Kroppenstedt R. M., Arun A. B., Lai W.-A., Shen F.-T., Rekha P. D., Young C. C. 2007; Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57:1131–1136 [CrossRef][PubMed]
    [Google Scholar]
  19. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef][PubMed]
    [Google Scholar]
  21. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef][PubMed]
    [Google Scholar]
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