1887

Abstract

Eight strains, isolated from necrotic pear blossoms in València, Spain, were compared with reference strains of and , both of which are pathogenic to species of pear tree, and to other species of the family using a polyphasic approach. Phenotypic analyses clustered the novel isolates into one phenon, distinct from other species of the genus , showing that the novel isolates constituted a homogeneous phenotypic group. Rep-PCR profiles, PCR products obtained with different pairs of primers and plasmid contents determined by restriction analysis showed differences between the novel strains and reference strains of and . Phylogenetic analysis of 16S rRNA, and gene sequences showed that the eight novel strains could not be assigned to any recognized species. On the basis of DNA–DNA hybridization studies, the novel isolates constituted a single group with relatedness values of 87–100 % to the designated type strain of the group, CFBP 5888. Depending on the method used, strain CFBP 5888 showed DNA–DNA relatedness values of between 22.7 and 50 % to strains of the closely related species and . The DNA G+C contents of two of the novel strains, CFBP 5888 and CFBP 5883, were 51.1 and 50.5 mol%, respectively. On the basis of these and previous results, the novel isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CFBP 5888 (=CECT 7348).

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2011-03-01
2020-01-21
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