1887

Abstract

A Gram-negative, motile, non-spore-forming bacterial strain, designated MJ07, was isolated from a farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ07 belongs to the family , class , and is related most closely to KCTC 22046 (98.6 % sequence similarity) and BN9 (96.9 %). The levels of 16S rRNA gene sequence similarity between strain MJ07 and members of all other recognized species of the family were below 95.2 %. The G+C content of the genomic DNA of strain MJ07 was 59.4 mol%. The detection of a quinone system with ubiquinone Q-8 as the major respiratory lipoquinone, putrescine as the predominant polyamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unknown aminolipids as major polar lipids and a fatty acid profile with C (32.0 %), C cyclo (24.7 %) and C cyclo 8 (11.5 %) as the major components supported the affiliation of strain MJ07 to the genus Strain MJ07 exhibited relatively low levels of DNA–DNA relatedness with respect to KCTC 22046 (50±8 %) and KACC 13183 (18±7 %). On the basis of its phenotypic and genotypic properties together with its phylogenetic distinctiveness, strain MJ07 (=KCTC 22455 =JCM 16386) should be classified in the genus as the type strain of a novel species, for which the name sp. nov. is proposed.

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2010-10-01
2019-10-18
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vol. , part 10, pp. 2326 - 2330

Two-dimensional TLC of polar lipids of strain MJ07 . Chloroform/methanol/water (65:25:4 by vol.) was used in the first direction, followed by chloroform/acetic acid/methanol/water (80:15:12:4 by vol.) in the second direction. For detection of total lipids, the plate was sprayed with 5 % ethanolic molybdatophosphoric acid. Molybdenum blue (Sigma) and ninhydrin were used for detection of phospholipids and aminolipids, respectively. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; AL1 and AL2, unidentified aminolipids; APL1 and APL6, unidentified aminophospholipids; L1 and L9–L12, unidentified polar lipids; PL7, unidentified phospholipid.



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