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Abstract

A Gram-staining-negative, strictly aerobic bacterium, designated strain SD10, was isolated from a tidal flat of the Yellow Sea, South Korea. Cells were non-spore-forming rods that showed catalase- and oxidase-positive reactions. Growth of strain SD10 was observed at 15–40 °C (optimum, 25–30 °C), at pH 6.0–9.0 (optimum, pH 6.5–8.5) and in the presence of 1–10 % (w/v) NaCl. Strain SD10 contained ubiquinone-10 (Q-10) as a major isoprenoid quinone and Cω7 (39.3 %), C (20.2 %), C (8.9 %) and Cω6 (8.1 %) as major fatty acids. The cellular polar lipids were identified as phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids. The G+C content of the genomic DNA was 55.2 mol%. Based on 16S rRNA gene sequence similarities, the strain was most closely related to Iso4 and P38, with similarities of 97.8 and 97.0 %, respectively. The DNA–DNA relatedness between strain SD10 and Iso4 was 12.0±3.2 %. A phylogenetic analysis based on 16S rRNA gene sequences showed that P38 and Iso4 formed a monophyletic cluster and that their 16S rRNA gene sequence similarity was 98.1 %. DNA–DNA hybridization between Iso4 and LMG 24711 was 22.9±2.7 %, indicating that the two strains belong to separate species. On the basis of chemotaxonomic data and molecular properties, we propose that strain SD10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SD10 ( = KACC 13700  = DSM 22014). In addition, we propose to transfer Lai 2009 to the genus as nom. nov. (type strain P38  = CCTCC AB 208227  = LMG 24711  = MCCC 1A01086), and we present an emended description of the genus .

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2011-04-01
2020-01-28
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Transmission electron micrograph showing the general morphology of a negatively stained cell of strain SD10 after growth for 3 days at 25 °C on marine agar. Bar, 0.5 µm.

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Polar lipids of strain SD10 separated by TLC and sprayed with molybdatophosphoric acid reagent. Ascending solvent systems: I, chloroform/methanol/water (65:25:4, by vol.); II, chloroform/acetic acid/methanol/water (80:13:10:2, by vol.). PG, Phosphatidylglycerol; MGDG, monoglycosyldiglyceride; GUDG, glucuronopyranosyldiglyceride, GL, unidentified glycolipids. For detection of polar lipids, molybdatophosphoric acid (PG, MGDG, GUDG, GL1 and GL2), ninhydrin (no detection), α-naphthol (MGDG, GUDG, GL1 and GL2) and Dittmer–Lester reagent (PG) were applied.

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Utilization of carbon sources by strain SD10 and related type strains determined using Biolog GN2 MicroPlates. [PDF](45 KB)

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