1887

Abstract

A bacterial strain (Chol-1S) that is able to oxidize cholesterol to CO and reduce nitrate to dinitrogen was enriched and isolated from an upflow sludge bed (USB) anoxic reactor that treats sanitary landfill leachate from the city of Montevideo, Uruguay. Cells of strain Chol-1S were Gram-negative, rod-shaped to slightly curved, measured 0·5–0·6×1·0-1·3 μm and were motile by a single polar flagellum. Strain Chol-1S grew optimally at 30–32 °C and pH 7·0, with a doubling time of 44–46 h when cholesterol was used as the sole carbon and energy source. The metabolism of strain Chol-1S was strictly respiratory, with oxygen or nitrate as the terminal electron acceptor. The presence of ubiquinone Q-8 as the sole respiratory lipoquinone indicated that strain Chol-1S belonged to the -subclass of the . Phosphatidylethanolamine was the predominant polar lipid and the G+C content of the DNA was 65·3 mol%. The fatty acid profile of strain Chol-1S, cultivated under denitrifying conditions by using a defined mineral medium supplemented with cholesterol, was characterized by the following major components: summed feature 4 (C 7 and/or iso C 2-OH), C, C 7 and hydroxy acid C 3-OH. Minor components included C, C, C, C, C, C, C 10-methyl and hydroxylated acids C 3-OH and C 3-OH. Analysis of the 16S rDNA sequence showed that strain Chol-1S represents a separate lineage within the , , and assemblage of the -. Strain Chol-1S had highest sequence similarity (96·5 %) with strain 72Chol, a denitrifying -. On the basis of polyphasic evidence, strain Chol-1S (=DSM 13999 =ATCC BAA-354) is proposed as the type strain of gen. nov., sp. nov.

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2003-07-01
2019-10-21
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