1887

Abstract

Members of the genus are responsible for cutaneous, pulmonary and disseminated human infections. From 2003 to 2008, four nocardioform strains (W8027, W8681, W9071 and W9241) were isolated from patients in the state of Florida, USA. Ribosomal gene sequencing analysis suggested that a novel species of the genus had been isolated. These strains were subjected to a taxonomic analysis using a polyphasic approach. Phenotypic analyses included morphological examination, biochemical profiling and antimicrobial susceptibility testing. Molecular studies included 16S rRNA and DNA gyrase B subunit ( gene sequence analyses and DNA–DNA hybridization. Phylogenetic neighbours were determined through 16S rRNA and gene sequence analyses. Phenotypic characteristics that differentiated the novel isolates from phylogenetically related species were growth at 45 °C, and three of the four novel strains utilized -rhamnose. The antimicrobial profiles could not reliably distinguish the novel species from related nocardiae. Analysis showed that the 16S rRNA gene sequences of the four novel isolates were identical. The analysis of the near full-length 16S rRNA gene showed 99.2 % sequence similarity to DSM 44729, DSM 44731 and JCM 10666, 98.7 % to DSM 45066, 98.2 % to JCM 12119 and 97.8 % to JCM 13313. Analysis of partial gene sequences showed that the novel isolates had 95.4 % similarity to DSM 44731, 95.3 % to DSM 44956, 94.4 % to JCM 12119, 93.8 % to DSM 44668, 93.5 % to DSM 45066, 93.4 % to JCM 10666 and 93.2 % to DSM 44729. The DNA–DNA relatedness values between the four novel strains were 86–89 %; the relatedness value for strain W9241 compared with JCM 10666 was 47 % and 46 % with DSM 44729, 44 % with DSM 44731, 32 % with DSM 45066 and 20 % with DSM 44668. The results of the taxonomic analysis suggested that the new isolates represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is W9241 (=DSM 45340=CCUG 57756).

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2011-02-01
2020-01-28
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References

  1. Baba, T., Nishiuchi, Y. & Yano, I. ( 1997; ). Composition of mycolic acid molecular species as a criterion in nocardial classification. Int J Syst Bacteriol 47, 795–801.[CrossRef]
    [Google Scholar]
  2. Berd, D. ( 1973; ). Laboratory identification of clinically important aerobic actinomycetes. Appl Microbiol 25, 665–681.
    [Google Scholar]
  3. Boiron, P., Provost, F., Chevrier, G. & Dupont, B. ( 1992; ). Review of nocardial infections in France 1987 to 1990. Eur J Clin Microbiol Infect Dis 11, 709–714.[CrossRef]
    [Google Scholar]
  4. Brenner, D. J., McWhorter, A. C., Knutson, J. K. & Steigerwalt, A. G. ( 1982; ). Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol 15, 1133–1140.
    [Google Scholar]
  5. Brown-Elliott, B. A., Brown, J. M., Conville, P. S. & Wallace, R. J., Jr ( 2006; ). Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy. Clin Microbiol Rev 19, 259–282.[CrossRef]
    [Google Scholar]
  6. Conville, P. S. & Witebsky, F. G. ( 2007; ). Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces, and other aerobic actinomycetes. In Manual of Clinical Microbiology, 9th edn, pp. 515–542. Edited by Murray, P. R., Baron, E. J., Jorgensen, J. H., Landry, M. L. & Pfaller, M. A.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  7. Goodfellow, M. ( 1998; ). Nocardia and related genera. In Topley and Wilson's Microbiology and Microbial Infections, 9th edn, vol. 2, Systematic Bacteriology, pp. 463–489. Edited by A. Balows & B. I. Duerden. London: Arnold.
  8. Goodfellow, M. & Maldonado, L. A. ( 2006; ). The families Dietziaceae, Gordoniaceae, Nocardiaceae and Tsukamurellaceae. In The Prokaryotes, 3rd edn, vol. 3<\emph>, pp. 843–888. Edited by Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. & Stackebrandt, E.. New York. : Springer.
    [Google Scholar]
  9. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Buczolits, S., Jäckel, U., Grün-Wollny, I. & Busse, H.-J. ( 2004; ). Nocardia tenerifensis sp. nov. Int J Syst Evol Microbiol 54, 381–383.[CrossRef]
    [Google Scholar]
  11. Klatte, S., Kroppenstedt, R. M. & Rainey, F. A. ( 1994; ). Rhodococcus opacus sp. nov., an unusual nutritionally versatile Rhodococcus-species. Syst Appl Microbiol 17, 355–360.[CrossRef]
    [Google Scholar]
  12. Kroppenstedt, R. M. ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef]
    [Google Scholar]
  13. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (SAB Technical Series no. 20) , pp. 173–199. Edited by Goodfellow, M. & Minnikin, D. E.. London. : Academic Press.
    [Google Scholar]
  14. Loeffelholz, M. J. & Scholl, D. R. ( 1989; ). Method for improved extraction of DNA from Nocardia asteroides. J Clin Microbiol 27, 1880–1881.
    [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Minnikin, D. E., Patel, P. V., Alshamaony, L. & Goodfellow, M. ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef]
    [Google Scholar]
  17. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  18. Morey, R. E., Galloway, R. L., Bragg, S. l., Steigerwalt, A. G., Mayer, L. W. & Levett, P. N. ( 2006; ). Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing. J Clin Microbiol 44, 3510–3516.[CrossRef]
    [Google Scholar]
  19. NCCLS ( 2003; ). Susceptibility testing of mycobacteria, nocardiae and other aerobic actinomycetes. Approved standard M24-A. Wayne, PA. : National Committee for Clinical Laboratory Standards.
    [Google Scholar]
  20. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S. ( 1955; ). The behaviour of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef]
    [Google Scholar]
  21. Roth, A., Andrees, S., Kroppenstedt, R. M., Harmsen, D. & Mauch, H. ( 2003; ). Phylogeny of the genus Nocardia based on reassessed 16S rRNA gene sequences reveals underspeciation and division of strains classified as Nocardia asteroides into three established species and two unnamed taxons. J Clin Microbiol 41, 851–856.[CrossRef]
    [Google Scholar]
  22. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16.
    [Google Scholar]
  23. Shen, F.-T., Lu, H. L., Lin, J. L., Huang, W. S., Arun, A. B. & Young, C. C. ( 2006; ). Phylogenetic analysis of members of the metabolically diverse genus Gordonia based on proteins encoding the gyrB gene. Res Microbiol 157, 367–375.[CrossRef]
    [Google Scholar]
  24. Stackebrandt, E., Frederiksen, W., Garrity, G., Grimont, P. A., Kämpfer, P., Maiden, M. C., Nesme, X., Rossella-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  25. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 438 - 442

Susceptibility testing results for patient isolates compared with those of some related type strains of the genus Fatty acid patterns (%). Extended neighbour-joining phylogenetic tree derived from aligned 16S rRNA gene sequences, showing the position of strain W9241 within the genus . Extended neighbour-joining phylogenetic tree derived from aligned gene gene sequences, showing the position of strain W9241 within the genus .

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