1887

Abstract

A Gram-negative, strictly aerobic and heterotrophic, non-spore-forming bacterial strain, designated LM22, was isolated from activated sludge of a sequencing batch reactor for the treatment of malachite green effluent. Cells of strain LM22 were slightly curved to straight rods (0.3–0.5×0.6–1.0 μm) and motile by a single polar flagellum. Strain LM22 was negative for oxidase and catalase activities and phototrophic growth. An internal membrane system and bacteriochlorophyll were absent. Growth occurred at 20–40 °C (optimum 30–35 °C) and pH 6.0–10.0 (optimum pH 7.0–7.5). Strain LM22 did not require NaCl for growth and tolerated up to 2.0 % NaCl (optimum 0.5 %). The major ubiquinone was Q-10. The major fatty acids (>10 % of the total) were C 7 (32.9 %), C cyclo 8 (18.7 %), C (12.1 %) and C 2-OH (10.5 %). Phylogenetic analysis of 16S rRNA gene sequences showed that AU0476 was the closest relative (90.4 % 16S rRNA gene sequence similarity). The DNA G+C content was 65.6 mol%. On basis of phenotypic, chemotaxonomic and phylogenetic data, strain LM22 was considered to represent a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is LM22 (=CGMCC 1.7660 =JCM 15798).

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2010-12-01
2024-03-29
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