1887

Abstract

A cholesterol side-chain-cleaving bacterial strain, AD-6, was isolated from fresh faeces of a clouded leopard () and was studied using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis showed that the novel strain formed a distinct subline within the genus , its closest neighbours being the type strains of , and , with sequence similarity values of 98.2, 97.8 and 97.6 %, respectively. The gene sequence of strain AD-6 exhibited similarities of 77–91 % with those of the type strains of recognized species of the genus , being most similar to the type strains of . , and (91, 87 and 84 % similarity, respectively). The results of whole-cell fatty acid analyses and DNA–DNA relatedness data readily distinguished the new isolate from its nearest neighbours. Strain AD-6 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AD-6 (=NRRL B-59395=CCTCC AB-209144).

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2011-01-01
2019-12-15
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vol. , part 1, pp. 165 - 169

Cellular fatty acid patterns that distinguish strain AD-6 from closely related species of the genus . Phylogenetic tree based on nearly complete 16S rRNA gene sequences (1417 nt) of all known members of the genus .

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