1887

Abstract

A cholesterol side-chain-cleaving bacterial strain, AD-6, was isolated from fresh faeces of a clouded leopard () and was studied using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis showed that the novel strain formed a distinct subline within the genus , its closest neighbours being the type strains of , and , with sequence similarity values of 98.2, 97.8 and 97.6 %, respectively. The gene sequence of strain AD-6 exhibited similarities of 77–91 % with those of the type strains of recognized species of the genus , being most similar to the type strains of . , and (91, 87 and 84 % similarity, respectively). The results of whole-cell fatty acid analyses and DNA–DNA relatedness data readily distinguished the new isolate from its nearest neighbours. Strain AD-6 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AD-6 (=NRRL B-59395=CCTCC AB-209144).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.020321-0
2011-01-01
2020-09-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/1/165.html?itemId=/content/journal/ijsem/10.1099/ijs.0.020321-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Bozzola J. J., Russell L. D. 1991 Electron Microscopy: Principles and Techniques for Biologists Boston: Jones & Bartlett;
    [Google Scholar]
  3. Collins M. D. 1985; Isoprenoid quinone analysis in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  6. Drzyzga O., Navarro L. J. M., Fernández L. H. L., García F. E., Perera J. 2009; Gordonia cholesterolivorans sp. nov., a cholesterol-degrading actinomycete isolated from sewage sludge. Int J Syst Evol Microbiol 59:1011–1015 [CrossRef]
    [Google Scholar]
  7. Franzetti A., Caredda P., Ruggeri C., La-Colla P., Tamburini E., Papacchini M., Bestetti G. 2009; Potential applications of surface active compounds by Gordonia sp. strain BS29 in soil remediation technologies. Chemosphere 75:801–807 [CrossRef]
    [Google Scholar]
  8. Hiraishi A. 1992; Direct automated sequencing of 16s rDNA amplified by polymerase chain reaction from bacterial culture without DNA purification. Lett Appl Microbiol 15:210–213 [CrossRef]
    [Google Scholar]
  9. Hiraishi A., Shin Y. K., Ueda Y., Sugiyama J. 1994; Automated sequencing of PCR-amplified 16S rRNA on ‘Hydrolink’ gels. J Microbiol Methods 19:145–154 [CrossRef]
    [Google Scholar]
  10. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  11. Kageyama A., Iida S., Yazawa K., Kudo T., Suzuki S., Koga T., Saito H., Inagawa H., Wada A. other authors 2006 Gordonia araii sp. nov. and Gordonia effusa sp. nov., isolated from patients in Japan. Int J Syst Evol Microbiol 56, 1817–1821 [CrossRef]
  12. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Goodfellow M. 1999; Gordonia desulfuricans sp. nov., a benzothiophene-desulphurizing actinomycete. Int J Syst Bacteriol 49:1845–1851 [CrossRef]
    [Google Scholar]
  13. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M. 2000; Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 50:2031–2036 [CrossRef]
    [Google Scholar]
  14. Kim K. K., Lee K. C., Klenk H.-P., Oh H. M., Lee J. S. 2009; Gordonia kroppenstedtii sp. nov., a phenol-degrading actinomycete isolated from a polluted stream. Int J Syst Evol Microbiol 59:1992–1996 [CrossRef]
    [Google Scholar]
  15. Kummer C., Schumann P., Stackebrandt E. 1999; Gordonia alkanivorans sp. nov., isolated from tar-contaminated soil. Int J Syst Bacteriol 49:1513–1522 [CrossRef]
    [Google Scholar]
  16. le Roes M., Goodwin C. M., Meyers P. R. 2008; Gordonia lacunae sp. nov., isolated from an estuary. Syst Appl Microbiol 31:17–23 [CrossRef]
    [Google Scholar]
  17. Linos A., Steinbüchel A., Spröer C., Kroppenstedt R. M. 1999; Gordonia polyisoprenivorans sp. nov., a rubber-degrading actinomycete isolated from an automobile tyre. Int J Syst Bacteriol 49:1785–1791 [CrossRef]
    [Google Scholar]
  18. Linos A., Berekaa M. M., Steinbüchel A., Kim K. K., Spröer C., Kroppenstedt R. M. 2002; Gordonia westfalica sp. nov., a novel rubber-degrading actinomycete. Int J Syst Evol Microbiol 52:1133–1139 [CrossRef]
    [Google Scholar]
  19. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  20. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Shen F. T., Lu H. L., Lin J. L., Huang W. S., Arun A. B., Young C. C. 2006; Phylogenetic analysis of members of the metabolically diverse genus Gordonia based on proteins encoding the gyrB gene. Res Microbiol 157:367–375 [CrossRef]
    [Google Scholar]
  23. Stackebrandt E., Smida J., Collins M. D. 1988; Evidence of phylogenetic heterogeneity within the genus Rhodococcus : revival of the genus Gordona (Tsukamura). J Gen Appl Microbiol 34:341–348 [CrossRef]
    [Google Scholar]
  24. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  25. Takeuchi M., Hatano K. 1998; Gordonia rhizosphera sp. nov. isolated from the mangrove rhizosphere. Int J Syst Bacteriol 48:907–912 [CrossRef]
    [Google Scholar]
  26. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  27. Tsukamura M. 1971; Proposal of a new genus, Gordona , for slightly acid-fast organisms occurring in sputa of patients with pulmonary disease and in soil. J Gen Microbiol 68:15–26 [CrossRef]
    [Google Scholar]
  28. Xue Y., Sun X., Zhou P., Liu R., Liang F., Ma Y. 2003; Gordonia paraffinivorans sp. nov., a hydrocarbon-degrading actinomycete isolated from an oil-producing well. Int J Syst Evol Microbiol 53:1643–1646 [CrossRef]
    [Google Scholar]
  29. Ye D. P., Lei J., Li W., Ge F. 2008; Purification and characterization of extracellular cholesterol oxidase from Enterobacter sp. World J Microbiol Biotechnol 24:2227–2233 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.020321-0
Loading
/content/journal/ijsem/10.1099/ijs.0.020321-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error