1887

Abstract

A novel actinomycete, designated strain Sco-B14, was isolated from volcanic ash collected near Darangshi Oreum (a parasitic or satellite volcano) in Jeju, Republic of Korea. The organism formed well-developed, branched substrate mycelium, on which short chains of non-motile spores were arranged singly or in clusters. Aerial mycelium was not produced. Globose bodies were observed. The reverse colour of colonies was light brown to brown. Diffusible pigments were produced on ISP medium 3 and oatmeal-nitrate agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Sco-B14 formed a lineage within the family and was distinct from established genera. The 16S rRNA gene sequence similarity of strain Sco-B14 to members of related genera of the family was 95.0–95.7 % to type strains of species, 94.7 % to IMSNU 22005, 94.7 % to K97-0003 and 94.0 % to LM 042. 3-Hydroxydiaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. Whole-cell sugars were glucose, rhamnose, ribose, xylose, arabinose, galactose and mannose. The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The menaquinone profile contained MK-10(H) (49 %), MK-9(H) (24 %), MK-10(H) (18 %) and MK-9(H) (9 %). The predominant fatty acids were iso-C and C. The DNA G+C content was 70.1 mol%. The combination of chemotaxonomic and phylogenetic data clearly separated the isolate from the type strains of all genera in the family . On the basis of the phylogenetic and chemotaxonomic data presented in this paper, strain Sco-B14 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is Sco-B14 (=KCTC 19661 =DSM 45362).

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2011-02-01
2020-01-29
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Phospholipid profile of strain Sco-B14 . (a) Mixture of standard phospholipids. (b) Phospholipid profile of strain Sco-B14 . DPG, Diphosphatidylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol.

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Cellular fatty acid compositions of strain Sco-B14 and its phylogenetically closest neighbours. [PDF](44 KB)

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