1887

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, heterotrophic, sulfur-oxidizing, non-motile, rod-shaped bacterium, designated KMM 3898, was isolated from an internal tissue of the sand snail , collected from the shallow sediments of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KMM 3898 formed a distinct phylogenetic lineage within the class and was most closely related to DSM 2157 (89.2 % 16S rRNA gene sequence similarity) and members of the genus (86.7–88.5 %). Chemotaxonomically, strain KMM 3898 contained the isoprenoid quinone Q-8, the polar lipids phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid and the fatty acids C 7, C 7 and C as predominant components (>10 %). The DNA G+C content of strain KMM 3898 was 43.4 mol%. On the basis of phenotypic features and phylogenetic analysis, strain KMM 3898 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is KMM 3898 (=NRIC 0757 =JCM 16494).

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2011-02-01
2020-01-29
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Two-dimensional thin-layer chromatogram of polar lipids of strain KMM 3898 . DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL, unknown phospholipid.

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Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing relationship of strain KMM 3898 with related members of the class . Accession numbers in parentheses. Bootstrap values (>70 %) based on 1000 replicates are shown at branch nodes.

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